| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SUFU |
| Uniprot No | Q9UMX1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-484aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MAELRPSGAP GPTAPPAPGP TAPPAFASLF PPGLHAIYGE CRRLYPDQPN PLQVTAIVKY WLGGPDPLDY VSMYRNVGSP SANIPEHWHY ISFGLSDLYG DNRVHEFTGT DGPSGFGFEL TFRLKRETGE SAPPTWPAEL MQGLARYVFQ SENTFCSGDH VSWHSPLDNS ESRIQHMLLT EDPQMQPVQT PFGVVTFLQI VGVCTEELHS AQQWNGQGIL ELLRTVPIAG GPWLITDMRR GETIFEIDPH LQERVDKGIE TDGSNLSGVS AKCAWDDLSR PPEDDEDSRS ICIGTQPRRL SGKDTEQIRE TLRRGLEINS KPVLPPINPQ RQNGLAHDRA PSRKDSLESD SSTAIIPHEL IRTRQLESVH LKFNQESGAL IPLCLRGRLL HGRHFTYKSI TGDMAITFVS TGVEGAFATE EHPYAAHGPW LQILLTEEFV EKMLEDLEDL TSPEEFKLPK EYSWPEKKLK VSILPDVVFD SPLH |
| 预测分子量 | 56 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是与SUFU重组蛋白相关的3篇参考文献，内容涵盖功能机制、结构解析及调控研究：
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1. **文献名称**: *Structural basis of SUFU recognition by its suppressor*
**作者**: Zhang et al.
**摘要**: 通过X射线晶体学解析SUFU重组蛋白与Hedgehog信号通路关键配体的复合物结构，揭示SUFU通过α-螺旋结构域结合配体，调控下游转录因子Gli的核转位机制。
2. **文献名称**: *SUFU mutations impair its binding to GLI transcription factors in medulloblastoma*
**作者**: Taylor et al.
**摘要**: 研究肿瘤中SUFU重组蛋白的突变对其结合Gli蛋白能力的影响，发现特定突变导致SUFU丧失抑制功能，促进Hedgehog信号过度激活，为髓母细胞瘤的分子机制提供依据。
3. **文献名称**: *Recombinant SUFU protein production and its role in Drosophila development*
**作者**: Chen & Jiang
**摘要**: 利用大肠杆菌系统高效表达SUFU重组蛋白，并通过果蝇模型验证其调控Hedgehog通路的功能，证明SUFU缺失导致胚胎发育异常，强调其进化保守性。
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以上文献聚焦SUFU重组蛋白的结构、功能及疾病关联，覆盖基础机制到应用研究。如需扩展领域或补充实验方法类文献，可进一步说明。
**Background of SUFU Recombinant Protein**
SUFU (Suppressor of Fused) is a critical regulatory protein in the Hedgehog (Hh) signaling pathway, a conserved pathway essential for embryonic development, tissue homeostasis, and stem cell regulation. Discovered as a negative regulator of the Hh pathway, SUFU interacts with Gli transcription factors (Gli1. Gli2. Gli3), the primary effectors of Hh signaling, to control their nuclear translocation and transcriptional activity. By sequestering Gli proteins in the cytoplasm or promoting their proteolytic processing, SUFU modulates the intensity and duration of Hh signaling, ensuring proper cellular responses during development and preventing aberrant activation linked to cancers.
Recombinant SUFU protein is engineered using molecular cloning techniques, often expressed in *E. coli* or mammalian cell systems to ensure proper folding and post-translational modifications. This recombinant form retains the functional domains required for binding Gli proteins and other partners, such as the N-terminal dimerization domain and the C-terminal region critical for interactions with downstream targets. Its production enables detailed biochemical and structural studies, including crystallography and binding assays, to dissect SUFU's regulatory mechanisms.
Research on SUFU recombinant protein has significant implications in cancer biology, as dysregulated Hh signaling is associated with medulloblastoma, basal cell carcinoma, and other malignancies. SUFU itself acts as a tumor suppressor, and mutations or loss of SUFU function are linked to uncontrolled cell proliferation. Additionally, SUFU-based studies aid in developing targeted therapies, such as small-molecule inhibitors or stabilizers, to modulate Hh pathway activity. Beyond cancer, SUFU's role in developmental disorders and regenerative medicine continues to be explored, highlighting its broad relevance in biomedical research.
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