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产品描述：人Klotho酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的KL含量。人Klotho蛋白是一种与衰老和多种生理过程相关的蛋白，其功能包括调节磷酸盐稳态、抑制相关信号通路、降低氧化应激并抑制炎症反应。研究发现，Klotho蛋白在多种肿瘤中发挥抑制因子作用，与肿瘤的发生发展及患者预后密切相关。Klotho蛋白通过抑制Insulin/IGF-1信号通路、诱导细胞凋亡、抑制EMT等机制发挥其抗肿瘤作用。试剂盒检测范围为0.156 ng/mL-10 ng/mL，适用于探究Klotho在疾病机制、药物干预效果或衰老相关研究中的表达变化；科研人员可通过分析不同生理状态下Klotho蛋白水平，为代谢性疾病、骨矿物质失调或神经退行性疾病的分子机制研究提供可靠工具，亦可应用于体外模型或动物实验中靶点表达量的动态监测。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Kl ELISA Kit; KLOT_HUMAN ELISA Kit; Klotho peptide ELISA Kit
缩写：KL
Uniprot No.：Q9UEF7
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Developmental Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human Klotho in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 89 | ||||
| Range % | 86-92 | |||||
| 1:2 | Average % | 98 | ||||
| Range % | 91-105 | |||||
| 1:4 | Average % | 96 | ||||
| Range % | 92-102 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 86-99 | |||||
回收率：
| The recovery of human Klotho spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 89-98 | ||||
| EDTA plasma (n=4) | 97 | 90-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 102.4912.4522.4722.350 52.1042.0762.0901.968 2.51.5041.5621.5331.411 1.250.8690.9310.9000.778 0.6250.4940.5070.5010.379 0.3120.2920.2790.2860.164 0.1560.1850.1790.1820.060 00.1260.1170.122 | ||||||
本试剂盒所含材料：
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A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human klotho antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled klotho antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
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