| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | ARCA1; C6orf98; CPG2; EDMD4; Enaptin; Myne-1; MYNE1; Nesp1; Nesprin-1; SCAR8; Syne-1; SYNE1;;Nesprin 1 |
| WB Predicted band size | Calculated MW: 1011 kDa ; Observed MW: 112 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human Nesprin 1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于Nesprin1抗体的3篇参考文献示例，包含虚构作者和摘要内容(仅供参考)：
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1. **文献名称**：*Nesprin1 anchors the cytoskeleton to the nuclear envelope in mammalian cells*
**作者**：Zhang Q. et al.
**摘要**：本研究通过免疫荧光和Western blot技术，利用特异性Nesprin1抗体揭示了其在核膜上的定位，证明Nesprin1通过连接核膜蛋白与细胞骨架维持细胞核结构稳定性，并影响细胞迁移能力。
2. **文献名称**：*Altered Nesprin1 expression in muscular dystrophy models*
**作者**：Lei M. et al.
**摘要**：该研究使用Nesprin1抗体检测肌肉组织样本，发现肌营养不良症模型中Nesprin1表达显著下调，提示其缺陷可能导致核膜脆性与肌肉功能异常。
3. **文献名称**：*Nesprin1 interacts with Emerin to regulate nuclear stiffness*
**作者**：Wilson R.H., Holaska J.M.
**摘要**：通过免疫共沉淀和抗体共定位实验，本文证实Nesprin1与Emerin在核膜形成复合物，共同调控细胞核机械应力响应，为核膜相关疾病的机制提供新见解。
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注：以上文献为示例，实际引用时需核实真实文献信息及作者名称。建议通过PubMed或Google Scholar搜索关键词“Nesprin1 antibody”获取最新研究。
Nesprin1 (nuclear envelope spectrin repeat protein 1), also known as SYNE1. is a key component of the LINC (linker of nucleoskeleton and cytoskeleton) complex, which bridges the nuclear envelope and connects the nuclear lamina to the cytoskeleton. It plays a critical role in maintaining nuclear structure, positioning, and mechanical signaling by anchoring the nucleus to actin filaments via its C-terminal KASH domain. Nesprin1 is highly expressed in muscle and neuronal tissues, where its function is vital for cellular integrity and mechanotransduction.
Antibodies targeting Nesprin1 are essential tools for studying its expression, localization, and interactions in cellular and disease models. They are widely used in techniques like immunofluorescence, Western blotting, and immunoprecipitation to explore Nesprin1's involvement in nuclear envelope stability, cell migration, and differentiation. Dysregulation or mutations in Nesprin1 are linked to muscular dystrophies (e.g., Emery-Dreifuss muscular dystrophy), cardiomyopathies, and neurological disorders, making these antibodies valuable in both basic research and clinical diagnostics.
Most Nesprin1 antibodies are raised against specific epitopes, such as its N-terminal spectrin repeats or C-terminal KASH domain, enabling isoform-specific detection. Validation often includes cross-reactivity checks across species (human, mouse, rat) and confirmation of specificity using knockout controls. Researchers rely on these antibodies to elucidate Nesprin1's role in cellular mechanobiology and its pathological implications, advancing insights into nuclear-cytoskeletal coupling mechanisms.
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