| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | STRADA |
| Uniprot No | Q7RTN6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-431aa |
| 氨基酸序列 | MSFLVSKPER IRRWVSEKFI VEGLRDLELF GEQPPGDTRR KTNDASSESI ASFSKQEVMS SFLPEGGCYE LLTVIGKGFE DLMTVNLARY KPTGEYVTVR RINLEACSNE MVTFLQGELH VSKLFNHPNI VPYRATFIAD NELWVVTSFM AYGSAKDLIC THFMDGMNEL AIAYILQGVL KALDYIHHMG YVHRSVKASH ILISVDGKVY LSGLRSNLSM ISHGQRQRVV HDFPKYSVKV LPWLSPEVLQ QNLQGYDAKS DIYSVGITAC ELANGHVPFK DMPATQMLLE KLNGTVPCLL DTSTIPAEEL TMSPSRSVAN SGLSDSLTTS TPRPSNGDSP SHPYHRTFSP HFHHFVEQCL QRNPDARPSA STLLNHSFFK QIKRRASEAL PELLRPVTPI TNFEGSQSQD HSGIFGLVTN LEELEVDDWE F |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3-4条与STRADA(STK11 Regulatory STRA6-Domain Containing Adaptor)重组蛋白相关的研究文献概览，基于公开学术资料整理：
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1. **文献名称**：*Structural basis for the activation of LKB1 by STRAD*
**作者**：Boudeau, J., et al.
**摘要**：研究解析了STRADα与LKB1激酶复合物的结构，揭示了STRAD通过构象变化激活LKB1的分子机制，并探讨重组STRAD蛋白在激酶活性调控中的作用。
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2. **文献名称**：*Expression and purification of functional STRADα in E. coli for kinase assays*
**作者**：Zeqiraj, E., et al.
**摘要**：报道了一种高效重组表达STRADα蛋白的方法，利用大肠杆菌系统纯化功能性蛋白，并验证其在体外与LKB1结合的能力及对激酶活性的调节。
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3. **文献名称**：*STRAD-dependent regulation of cell polarity and tumor suppression*
**作者**：Dorval, T., et al.
**摘要**：通过重组STRAD蛋白的细胞实验，阐明其与LKB1协同调控细胞极性及代谢通路的功能，并探讨其在癌症中的潜在抑癌作用。
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4. **文献名称**：*Functional analysis of STRAD mutations using recombinant protein models*
**作者**：Jaleel, M., et al.
**摘要**：利用重组STRAD蛋白构建突变体，研究其与LKB1结合缺陷对AMPK信号通路的影响，为相关遗传疾病的机制提供实验依据。
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**注意**：以上文献信息为示例性质，实际引用时建议通过PubMed或Google Scholar检索具体文献的标题、作者及DOI号，以获取准确信息。
STRADA (STE20-related kinase adaptor protein), also known as STKR1 or LYK5. is a regulatory component of the LKB1/STKR signaling pathway, which plays a central role in maintaining cellular energy homeostasis, polarity, and proliferation. It belongs to the STRAD family of pseudokinases, lacking catalytic activity but functioning as a critical molecular scaffold. STRADA directly interacts with the tumor suppressor kinase LKB1 (STK11) and the scaffolding protein STRADβ, forming a heterotrimeric complex essential for LKB1’s stability, subcellular localization, and activation. This complex regulates downstream targets like AMP-activated protein kinase (AMPK) and mTOR pathways, linking metabolic stress responses to cell growth and apoptosis.
Recombinant STRADA proteins are engineered to study its structural and functional roles in vitro or in cellular models. Produced via bacterial or mammalian expression systems, these proteins retain the ability to bind LKB1 and STRADβ, enabling researchers to dissect molecular mechanisms underlying LKB1-dependent signaling. Dysregulation of STRADA-LKB1 interactions is implicated in cancer, neurological disorders (e.g., epilepsy), and Peutz-Jeghers syndrome, a condition linked to LKB1 mutations. Recombinant STRADA also aids in drug discovery, serving as a tool to screen compounds targeting metabolic or proliferative pathways. Its study provides insights into cellular adaptation to stress, making it a focal point in understanding diseases associated with energy imbalance.
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