| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | STOM |
| Uniprot No | P27105 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-288aa |
| 氨基酸序列 | MAEKRHTRDSEAQRLPDSFKDSPSKGLGPCGWILVAFSFLFTVITFPISIWMCIKIIKEYERAIIFRLGRILQGGAKGPGLFFILPCTDSFIKVDMRTISFDIPPQEILTKDSVTISVDGVVYYRVQNATLAVANITNADSATRLLAQTTLRNVLGTKNLSQILSDREEIAHNMQSTLDDATDAWGIKVERVEIKDVKLPVQLQRAMAAEAEASREARAKVIAAEGEMNASRALKEASMVITESPAALQLRYLQTLTTIAAEKNSTIVFPLPIDMLQGIIGAKHSHLG |
| 预测分子量 | 47.7kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为与STOM(Stomatin)重组蛋白相关的参考文献示例(内容基于研究方向虚构，实际文献需根据具体数据库检索)：
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1. **文献名称**: *"Functional characterization of recombinant Stomatin in red blood cell membrane organization"*
**作者**: Müller A, et al.
**摘要**: 研究通过大肠杆菌系统表达重组STOM蛋白，分析其与红细胞膜脂筏的相互作用，发现STOM通过调控离子通道活性维持细胞膜稳定性。
2. **文献名称**: *"Crystallographic analysis of human Stomatin reveals a novel oligomerization domain"*
**作者**: Zhang Y, et al.
**摘要**: 利用昆虫细胞系统表达并纯化人源STOM重组蛋白，通过X射线晶体学解析其三维结构，揭示其寡聚化结构域对机械信号感知的潜在作用。
3. **文献名称**: *"Recombinant Stomatin alleviates neuronal hyperexcitability in a Drosophila epilepsy model"*
**作者**: Lee S, et al.
**摘要**: 在果蝇癫痫模型中验证重组STOM蛋白的治疗潜力，发现其通过调节TRP通道活性减少神经元过度兴奋。
4. **文献名称**: *"Expression optimization and purification of Stomatin in mammalian cells for drug screening"*
**作者**: Gupta R, et al.
**摘要**: 开发基于HEK293细胞的重组STOM蛋白高效表达与纯化方案，并应用于高通量药物筛选，以靶向STOM相关代谢疾病。
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**注意**：以上为模拟示例，实际文献需通过PubMed、Web of Science等平台检索关键词(如“Stomatin recombinant expression”、“STOM protein purification”)获取。建议结合具体研究领域细化检索策略。
STOM (stomatin) is a highly conserved membrane-associated protein first identified in human erythrocytes, where it plays a critical role in regulating ion channel activity and maintaining membrane mechanical properties. This 31-35 kDa protein belongs to the stomatin-prohibitin-flotillin-HflK/C (SPFH) domain family, characterized by a conserved hydrophobic domain involved in lipid raft association. Structurally, STOM lacks transmembrane domains but anchors to membranes via palmitoylation and hydrophobic interactions, preferentially localizing to cholesterol-rich microdomains.
Functionally, STOM acts as a scaffolding protein that modulates various membrane transporters and channels, particularly mechanosensitive ion channels like ENaC (epithelial sodium channel) and acid-sensing ion channels (ASICs). Its ability to influence membrane stiffness and curvature sensing contributes to cellular responses in mechanotransduction. Research has linked STOM deficiencies to hematological disorders including hereditary stomatocytosis, where red blood cells exhibit abnormal permeability to cations and altered deformability. Beyond erythrocytes, STOM is ubiquitously expressed in diverse tissues including kidney, neurons, and immune cells, suggesting broader physiological roles in cellular homeostasis, sensory perception, and vesicle trafficking.
Recombinant STOM proteins have become essential tools for studying these mechanisms. Produced through bacterial (E. coli) or eukaryotic expression systems, recombinant STOM retains its oligomerization capacity and membrane-binding properties when properly refolded. Current applications include structural studies using cryo-EM to elucidate its interaction with lipid bilayers, functional assays examining ion channel regulation, and diagnostic development for membrane-related disorders. Recent interest focuses on its potential as a therapeutic target for neuropathic pain management and degenerative conditions involving mechanosensitive pathway dysregulation.
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