| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | STMN3 |
| Uniprot No | Q9NZ72 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 39-180aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MDMEVKQLDK RASGQSFEVI LKSPSDLSPE SPMLSSPPKK KDTSLEELQK RLEAAEERRK TQEAQVLKQL AERREHEREV LHKALEENNN FSRQAEEKLN YKMELSKEIR EAHLAALRER LREKELHAAE VRRNKEQREE MSG |
| 预测分子量 | 19 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于STMN3(Stathmin3)重组蛋白的模拟参考文献示例。由于实际文献可能存在差异，建议通过学术数据库(如PubMed、Google Scholar)进一步核实：
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1. **标题**: "Production and Functional Characterization of Recombinant Human STMN3 Protein"
**作者**: Smith A, et al.
**摘要**: 本研究报道了在大肠杆菌中高效表达并纯化重组人STMN3蛋白的方法，证实其通过调控微管动力学抑制微管聚合，并可用于体外细胞迁移实验的功能分析。
2. **标题**: "STMN3 Recombinant Protein Attenuates Neuronal Degeneration in a Parkinson’s Disease Model"
**作者**: Li X, et al.
**摘要**: 通过体外递送重组STMN3蛋白至多巴胺能神经元，发现其通过稳定微管结构减少α-突触核蛋白毒性，为神经退行性疾病治疗提供潜在策略。
3. **标题**: "Structural Insights into STMN3’s Microtubule-Destabilizing Activity Using Recombinant Protein Crystallography"
**作者**: Chen H, et al.
**摘要**: 利用重组STMN3蛋白解析其晶体结构，揭示其与微管蛋白结合的特定结构域，阐明其破坏微管组装的分子机制。
4. **标题**: "Optimization of STMN3 Recombinant Expression in Mammalian Cells for Functional Screening"
**作者**: Wang Y, et al.
**摘要**: 开发了哺乳动物细胞系统中重组STMN3的可溶性表达体系，验证其在药物筛选模型中对微管动态调节的高通量应用价值。
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**注意**：以上内容为模拟生成的示例，实际文献需通过学术数据库检索。若需具体文献，建议结合关键词(如 "STMN3 recombinant protein" + "microtubule")在PubMed或Web of Science中查询。
STMN3 (Stathmin-3), also known as SCG10. is a member of the stathmin family of cytosolic phosphoproteins that play critical roles in regulating microtubule dynamics. It is encoded by the *STMN3* gene and is predominantly expressed in neuronal tissues, where it contributes to neural development, synaptic plasticity, and intracellular transport. Structurally, STMN3 contains a conserved stathmin-like domain with microtubule-destabilizing activity, mediated through its ability to bind tubulin heterodimers and promote microtubule disassembly. Unlike its paralog STMN1 (stathmin-1), STMN3 features an N-terminal palmitoylation signal that targets it to Golgi-derived vesicles, enabling localized regulation of microtubule remodeling in specific cellular compartments.
Functionally, STMN3 is regulated by phosphorylation in response to extracellular signals, such as growth factors, which modulate its interaction with microtubules. Dysregulation of STMN3 has been implicated in neurodegenerative disorders, including Alzheimer’s disease, where aberrant microtubule dynamics disrupt axonal transport. Additionally, STMN3 overexpression has been observed in certain cancers, linking it to tumor cell migration and metastasis. Recombinant STMN3 protein, typically produced in *E. coli* or mammalian expression systems, retains its tubulin-binding capacity and is widely used in biochemical assays to study microtubule polymerization kinetics, protein-protein interactions, and signaling pathways. Its purity and activity are validated via SDS-PAGE, Western blotting, and functional assays. Research utilizing recombinant STMN3 has provided insights into neurodevelopment mechanisms and potential therapeutic strategies targeting microtubule instability in disease contexts.
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