| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | STK3 |
| Uniprot No | Q13188 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-491aa |
| 氨基酸序列 | EQPPAPKSKLKKLSEDSLTKQPEEVFDVLEKLGEGSYGSVFKAIHKESGQ VVAIKQVPVESDLQEIIKEISIMQQCDSPYVVKYYGSYFKNTDLWIVMEY CGAGSVSDIIRLRNKTLIEDEIATILKSTLKGLEYLHFMRKIHRDIKAGN ILLNTEGHAKLADFGVAGQLTDTMAKRNTVIGTPFWMAPEVIQEIGYNCV ADIWSLGITSIEMAEGKPPYADIHPMRAIFMIPTNPPPTFRKPELWSDDF TDFVKKCLVKNPEQRATATQLLQHPFIKNAKPVSILRDLITEAMEIKAKR HEEQQRELEEEEENSDEDELDSHTMVKTSVESVGTMRATSTMSEGAQTMI EHNSTMLESDLGTMVINSEDEEEEDGTMKRNATSPQVQRPSFMDYFDKQD FKNKSHENCNQNMHEPFPMSKNVFPDNWKVPQDGDFDFLKNLSLEELQMR LKALDPMMEREIEELRQRYTAKRQPILDAMDAKKRRQQNF |
| 预测分子量 | 78 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于STK3(MST2)重组蛋白的3篇代表性文献示例(注：文献信息为模拟概括，仅供参考)：
1. **文献名称**：*"Structural and Functional Analysis of the MST2 Kinase Domain in Complex with Its Inhibitor"*
**作者**：Zhang Y et al.
**摘要**：通过重组表达人源STK3/MST2激酶结构域，解析其与抑制剂复合物的晶体结构，揭示了ATP结合口袋的关键相互作用，为靶向Hippo通路的药物设计提供依据。
2. **文献名称**：*"Recombinant MST2 Kinase Production and Its Role in Apoptosis Regulation"*
**作者**：Lee JH et al.
**摘要**：利用昆虫细胞系统表达重组STK3蛋白，证明其磷酸化底物MOB1并激活下游促凋亡信号，阐明了STK3在细胞死亡中的调控机制。
3. **文献名称**：*"Optimization of Expression and Purification of Active Human MST2 in E. coli for High-Throughput Screening"*
**作者**：Chen X et al.
**摘要**：开发了一种大肠杆菌表达系统，通过优化条件获得高活性重组STK3蛋白，并应用于激酶抑制剂的高通量筛选，验证了多个潜在化合物。
**备注**：实际文献需通过PubMed、Web of Science等平台检索关键词“STK3 recombinant”“MST2 expression”获取。部分研究可能涉及STK3的同源蛋白或复合物功能分析。
**Background of STK3 Recombinant Protein**
STK3 (serine/threonine-protein kinase 3), also known as MST2. is a key component of the Hippo signaling pathway, which regulates organ size, cell proliferation, apoptosis, and tissue homeostasis. It belongs to the STE20-like kinase family and shares structural and functional similarities with STK4 (MST1). STK3 acts as a tumor suppressor by phosphorylating downstream effectors, such as LATS1/2. to inhibit YAP/TAZ transcriptional coactivators, thereby controlling cell growth and preventing cancer progression.
Recombinant STK3 protein is engineered in vitro using expression systems like *E. coli* or mammalian cells, ensuring high purity and biological activity. It typically retains the kinase domain and regulatory regions necessary for interactions with binding partners (e.g., SAV1) and substrate phosphorylation. Researchers use recombinant STK3 to study Hippo pathway dynamics, screen for kinase inhibitors, or explore its role in diseases linked to pathway dysregulation, such as cancers, fibrosis, and neurodegenerative disorders.
Dysregulation of STK3 is associated with tumorigenesis, metastasis, and poor prognosis. Its recombinant form enables mechanistic studies, including phosphorylation assays, structural analysis, and therapeutic target validation. Additionally, STK3 has emerging roles in immune regulation and metabolic processes, broadening its relevance in biomedical research.
In summary, STK3 recombinant protein serves as a critical tool for dissecting Hippo signaling mechanisms and developing targeted therapies for diseases driven by pathway imbalances. Its applications span basic research, drug discovery, and translational studies.
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