| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | STEAP4 |
| Uniprot No | Q687X5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-152aa |
| 氨基酸序列 | MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMEKT CIDALPLTMN SSEKQETVCI FGTGDFGRSL GLKMLQCGYS VVFGSRNPQKTTLLPSGAEV LSYSEAAKKS GIIIIAIHRE HYDFLTELTE VLNGKILVDI SNNLKINQYP ESNAEYLAHL VPGAHVVKAF NTISAWALQS GALDASRQ |
| 预测分子量 | 21 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于STEAP4重组蛋白的3篇示例参考文献(注：文献信息为模拟示例，非真实存在)：
1. **文献名称**：*"STEAP4 regulates inflammatory response in adipocytes via its recombinant protein expression"*
**作者**：Li, X., Wang, Y., & Zhang, H.
**摘要**：研究通过重组表达人源STEAP4蛋白，发现其通过调控NF-κB信号通路抑制脂肪细胞中炎症因子(如TNF-α、IL-6)的释放，提示其在代谢性炎症中的潜在作用。
2. **文献名称**：*"Recombinant STEAP4 protein ameliorates insulin resistance in high-fat diet-induced obese mice"*
**作者**：Kim, S., Park, J., & Lee, M.
**摘要**：利用大肠杆菌系统表达纯化STEAP4重组蛋白，并在肥胖小鼠模型中验证其通过增强胰岛素受体敏感性改善胰岛素抵抗的作用机制。
3. **文献名称**：*"Structural characterization and functional analysis of the metalloreductase domain in STEAP4 recombinant protein"*
**作者**：Garcia, R., et al.
**摘要**：解析STEAP4重组蛋白的金属还原酶结构域晶体结构，并通过体外实验证实其铁离子还原活性对细胞铁代谢和氧化应激的调控功能。
4. **文献名称**：*"STEAP4 recombinant protein suppresses prostate cancer cell proliferation via modulating ERK signaling"*
**作者**：Chen, T., et al.
**摘要**：研究利用真核表达系统获得功能性STEAP4重组蛋白，并证明其通过抑制ERK磷酸化抑制前列腺癌细胞增殖和迁移。
(注：如需真实文献，建议通过PubMed或Google Scholar搜索关键词“STEAP4 recombinant protein”获取最新研究。)
**Background of STEAP4 Recombinant Protein**
STEAP4 (Six-transmembrane epithelial antigen of the prostate 4), also known as TNFAIP9. is a member of the STEAP family of metalloreductases, which includes four homologs (STEAP1-4). These transmembrane proteins are implicated in metal ion metabolism, redox reactions, and cellular signaling. STEAP4. located on human chromosome 7 (7q21.13), encodes a six-transmembrane domain protein with both N- and C-termini oriented intracellularly. It is widely expressed in metabolic tissues, including adipose tissue, liver, and pancreas, and is strongly associated with metabolic regulation.
STEAP4 functions as a ferric/cupric reductase, facilitating the reduction of Fe³⁺ and Cu²⁺, which is critical for cellular iron/copper homeostasis. It also modulates oxidative stress and inflammatory responses via interactions with pathways like TNF-α/NF-κB and insulin signaling. Research links STEAP4 to metabolic disorders such as obesity, type 2 diabetes, and insulin resistance. Its expression is upregulated in adipose tissue under obese conditions, suggesting a compensatory role in mitigating metabolic inflammation and improving insulin sensitivity.
Recombinant STEAP4 protein is engineered using bacterial or mammalian expression systems to study its biochemical properties, enzymatic activity, and interactions. This protein serves as a tool to explore STEAP4's role in disease mechanisms, including its dual function in cancer (pro-tumor or anti-tumor effects depending on context) and metabolic dysregulation. Additionally, it aids in developing therapeutic strategies targeting metabolic syndromes or malignancies.
Overall, STEAP4 represents a critical nexus between metal homeostasis, redox balance, and metabolic health, making its recombinant form valuable for both basic research and translational applications.
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