| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | STK11 |
| Uniprot No | Q15831 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-430aa |
| 氨基酸序列 | MEVVDPQQLGMFTEGELMSVGMDTFIHRIDSTEVIYQPRRKRAKLIGKYLMGDLLGEGSYGKVKEVLDSETLCRRAVKILKKKKLRRIPNGEANVKKEIQLLRRLRHKNVIQLVDVLYNEEKQKMYMVMEYCVCGMQEMLDSVPEKRFPVCQAHGYFCQLIDGLEYLHSQGIVHKDIKPGNLLLTTGGTLKISDLGVAEALHPFAADDTCRTSQGSPAFQPPEIANGLDTFSGFKVDIWSAGVTLYNITTGLYPFEGDNIYKLFENIGKGSYAIPGDCGPPLSDLLKGMLEYEPAKRFSIRQIRQHSWFRKKHPPAEAPVPIPPSPDTKDRWRSMTVVPYLEDLHGADEDEDLFDIEDDIIYTQDFTVPGQVPEEEASHNGQRRGLPKAVCMNGTEAAQLSTKSRAEGRAPNPARKACSASSKIRRLSAC |
| 预测分子量 | 64.3kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于STK11(LKB1)重组蛋白研究的模拟参考文献示例(文献名为假设，供参考)：
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1. **文献名称**：*"Recombinant STK11 Kinase Domain Production and Its Role in AMPK Pathway Activation"*
**作者**：Zhang Y. et al.
**摘要**：研究报道了通过大肠杆菌系统表达并纯化STK11激酶结构域重组蛋白，验证其体外磷酸化AMPK的能力，揭示了STK11在能量代谢调控中的分子机制。
2. **文献名称**：*"Structural Analysis of STK11/STRAD/MO25 Ternary Complex Using Recombinant Proteins"*
**作者**：Sato T. et al.
**摘要**：利用重组STK11、STRAD和MO25蛋白共表达技术，解析了三者复合物的晶体结构，阐明了STK11活性调控依赖STRAD/MO25结合的分子基础。
3. **文献名称**：*"Functional Rescue of STK11 Cancer Mutants via Recombinant Protein Reconstitution"*
**作者**：Guo L. et al.
**摘要**：通过构建STK11常见肿瘤突变体的重组蛋白，发现部分突变可通过辅助分子伴侣恢复激酶活性，为靶向STK11失活的治疗策略提供依据。
4. **文献名称**：*"High-Yield Purification of Active STK11 Recombinant Protein for Drug Screening Assays"*
**作者**：Martinez R. et al.
**摘要**：开发了基于昆虫细胞表达系统的STK11重组蛋白高效纯化方案，并应用于激酶抑制剂高通量筛选，鉴定出新型小分子调节剂。
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**备注**：以上文献为示例，实际引用时需通过学术数据库(如PubMed、Web of Science)检索真实文献。建议使用关键词“STK11 recombinant protein expression”或“LKB1 purification”进行精准查询。
STK11 (serine/threonine kinase 11), also known as LKB1. is a tumor suppressor gene encoding a ubiquitously expressed kinase critical for regulating cell polarity, metabolism, and proliferation. Discovered in 1998 through its linkage to Peutz-Jeghers syndrome (PJS), an inherited disorder characterized by gastrointestinal polyposis and increased cancer risk, STK11 mutations are also implicated in sporadic cancers, including lung, cervical, and pancreatic malignancies. The protein functions as a master kinase, directly activating AMPK (AMP-activated protein kinase) and other AMPK-related kinases to coordinate cellular responses to energy stress, nutrient availability, and microenvironmental cues.
Recombinant STK11 protein refers to the engineered version produced in vitro using expression systems like *E. coli* or mammalian cells. This bioengineered protein retains key functional domains, including the kinase domain and regulatory regions necessary for interactions with binding partners like STRAD and MO25. Researchers utilize recombinant STK11 to study its enzymatic activity, substrate specificity, and structural biology, particularly in reconstituting signaling pathways like the LKB1-AMPK axis. It also serves as a tool for drug discovery, enabling high-throughput screening for compounds that modulate kinase activity or restore function in STK11-deficient cancers.
Current studies focus on understanding how post-translational modifications (e.g., phosphorylation) or cancer-associated mutations alter STK11's tumor-suppressive functions. The recombinant protein’s availability accelerates mechanistic insights into metabolic dysregulation in tumors and supports therapeutic strategies targeting STK11 downstream effectors. Despite challenges in maintaining native conformation during production, advancements in protein engineering continue to enhance its utility in both basic research and translational applications.
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