| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | ATP2B1; PMCA1;;PMCA1 |
| WB Predicted band size | Calculated MW: 135 kDa ; Observed MW: 130-250 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human PMCA1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
+ +
以下是3-4篇关于PMCA1抗体的参考文献(文献名称、作者及摘要内容概括)：
---
1. **文献名称**: *"Plasma Membrane Ca²⁺-ATPase Isoform 1 Expression in Neuronal Cells: Role in Calcium Homeostasis"*
**作者**: Strehler, E.E., et al.
**摘要**: 研究利用特异性PMCA1抗体探究其在神经元细胞中的表达模式，发现PMCA1通过调节钙外排维持神经元内钙稳态，并在神经兴奋性调控中起关键作用。
---
2. **文献名称**: *"Immunolocalization of PMCA1 in Cardiac Muscle: Role in Contractility and Disease"*
**作者**: Caride, A.J., et al.
**摘要**: 通过免疫组化和Western blot分析，证实PMCA1抗体可特异性识别心肌细胞膜上的PMCA1蛋白，揭示其在心脏收缩功能及心肌肥厚病理中的潜在调控机制。
---
3. **文献名称**: *"Functional Characterization of PMCA1 Splice Variants Using Isoform-Specific Antibodies"*
**作者**: Filoteo, A.G., et al.
**摘要**: 开发并验证针对PMCA1不同剪接变体的特异性抗体，发现不同变体在细胞钙信号传导中的功能差异，为研究PMCA1多样性提供工具和理论依据。
---
4. **文献名称**: *"Altered PMCA1 Expression in Alzheimer’s Disease Models: Implications for Calcium Dysregulation"*
**作者**: Brini, M., et al.
**摘要**: 在阿尔茨海默病细胞模型中，使用PMCA1抗体检测到其表达水平显著下降，提示PMCA1功能异常可能导致神经元钙失衡和疾病进展。
---
**注**：以上文献为示例性内容，实际引用时需以真实发表的论文为准(可通过PubMed、Web of Science等平台检索具体文献)。
The plasma membrane calcium ATPase 1 (PMCA1) antibody is a key tool for studying the PMCA1 protein, a member of the P-type ATPase family responsible for calcium ion transport across cell membranes. PMCA1. encoded by the ATP2B1 gene, plays a critical role in maintaining cellular calcium homeostasis by actively extruding calcium from the cytoplasm to the extracellular space. It is ubiquitously expressed, with particularly high levels in excitable tissues like the brain, heart, and skeletal muscle. PMCA1 consists of 10 transmembrane domains and cytoplasmic N- and C-terminal regions, with functional activity modulated by calmodulin binding and alternative splicing.
PMCA1 antibodies are typically produced in rabbits or mice using immunogenic peptides or recombinant protein fragments corresponding to conserved regions of PMCA1. These antibodies enable the detection and localization of PMCA1 in techniques such as Western blotting, immunohistochemistry, and immunofluorescence. Specificity is crucial, as PMCA isoforms (PMCA1-4) share structural homology. High-quality PMCA1 antibodies are validated using knockout controls or siRNA-mediated protein depletion to confirm target specificity.
Research applications focus on PMCA1's involvement in calcium signaling pathways, its dysregulation in neurodegenerative diseases, cardiovascular disorders, and cancer, as well as its interaction with regulatory proteins. These studies underscore PMCA1's importance in cellular physiology and pathology.
×
