| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | DER12; ei-type; HNP36; Nucleoside transporter; SLC29A2;;ENT2 |
| WB Predicted band size | Calculated MW: 50 kDa ; Observed MW: 55-65 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human ENT2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于ENT2抗体的3篇参考文献示例(注：以下内容为虚构示例，实际文献需通过学术数据库检索确认)：
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1. **文献名称**：*Characterization of ENT2-Specific Antibodies for Detection of Nucleoside Transporter Expression in Cancer Cells*
**作者**：Smith A, et al.
**摘要**：研究开发了高特异性ENT2抗体，用于检测多种癌细胞系中ENT2蛋白的表达水平，证实其在化疗药物转运中的潜在作用。
2. **文献名称**：*Role of ENT2 in Adenosine Uptake: Validation by CRISPR and Antibody-Based Approaches*
**作者**：Chen L, et al.
**摘要**：通过CRISPR基因编辑和ENT2抗体免疫印迹，验证了ENT2在调控细胞外腺苷摄取中的关键功能，及其在炎症反应中的意义。
3. **文献名称**：*Comparative Study of ENT1 and ENT2 Antibodies in Neuronal Tissue Localization*
**作者**：Wang X, et al.
**摘要**：对比ENT1/ENT2抗体的免疫组化结果，发现ENT2在小脑神经元中的高表达，提示其可能参与神经保护机制。
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如需真实文献，建议通过PubMed或Google Scholar搜索关键词(如"ENT2 antibody"、"SLC29A2" + "antibody")，筛选近年发表的实验研究或抗体验证类论文。
The ENT2 antibody is designed to detect equilibrative nucleoside transporter 2 (ENT2. also known as SLC29A2), a member of the solute carrier 29 family. ENT2 is a ubiquitously expressed transmembrane protein that facilitates bidirectional transport of nucleosides and nucleobases across cell membranes, depending on concentration gradients. It plays a critical role in cellular nucleoside homeostasis, nucleotide synthesis, and the uptake of nucleoside-derived antiviral or anticancer drugs (e.g., gemcitabine). Unlike ENT1. which is sensitive to inhibition by nanomolar concentrations of nitrobenzylthioinosine (NBMPR), ENT2 is NBMPR-insensitive and exhibits broader substrate specificity, including transporting deoxyribonucleosides.
ENT2 is highly expressed in tissues such as skeletal muscle, heart, brain, and placenta. Its dysfunction has been linked to pathologies like mitochondrial disorders, neurodegenerative diseases, and chemotherapy resistance. Research on ENT2 often focuses on its role in drug transport efficiency, cellular energy metabolism, and neurological protection.
The ENT2 antibody is widely used in Western blotting, immunohistochemistry, and immunofluorescence to study protein localization, expression levels, and regulatory mechanisms under physiological or pathological conditions. Validated antibodies help clarify ENT2's tissue-specific functions and its potential as a therapeutic target in cancer or neurological conditions.
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