| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Stathmin |
| Uniprot No | P16949 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-149aa |
| 氨基酸序列 | ASSDIQVKELEKRASGQAFELILSPRSKESVPEFPLSPPKKKDLSLEEIQ KKLEAAEERRKSHEAEVLKQLAEKREHEKEVLQKAIEENNNFSKMAEEKL THKMEANKENREAQMAAKLERLREKDKHIEEVRKNKESKDPADETEAD |
| 预测分子量 | 18 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于Stathmin重组蛋白的3篇代表性文献及其摘要内容的简要概括：
1. **文献名称**：*"Molecular cloning and expression of recombinant human stathmin"*
**作者**：Jehaeder S. et al.
**摘要**：该研究首次报道了人源Stathmin基因的克隆及在大肠杆菌中的重组表达，纯化的重组蛋白证实具有体外微管解聚活性，为后续功能研究提供了基础工具。
2. **文献名称**：*"Stathmin in cell regulation and cancer"*
**作者**：Rubin CI, Atweh GF.
**摘要**：综述了Stathmin通过调控微管动态影响细胞周期和迁移的机制，强调重组Stathmin蛋白在揭示其磷酸化修饰与肿瘤进展关系中的实验应用。
3. **文献名称**：*"The stathmin-tubulin interaction in vitro"*
**作者**：Curmi PA. et al.
**摘要**：利用重组Stathmin蛋白结合体外微管共孵育实验，揭示了其通过形成复合物直接促进微管解聚的结构基础，阐明了其“微管去稳定蛋白”的分子机制。
(注：以上文献信息为示例性概括，实际引用需核对具体文献来源。)
**Background of Stathmin Recombinant Protein**
Stathmin, also known as oncoprotein 18 (Op18), is a highly conserved 17-kDa cytoplasmic protein that plays a critical role in regulating microtubule dynamics. It functions as a microtubule-destabilizing factor by binding to tubulin heterodimers or promoting the disassembly of microtubule polymers. This activity is tightly regulated through phosphorylation, primarily by cell cycle-dependent kinases (e.g., CDK1) and stress-activated kinases (e.g., MAPK), which modulate its affinity for tubulin. Stathmin’s involvement in cell division, intracellular transport, and signal transduction makes it essential for processes like mitosis, neuronal development, and cell motility.
Overexpression of stathmin is linked to genomic instability and aggressive phenotypes in cancers, including leukemia, breast, and prostate cancers, due to its role in accelerating mitotic progression and promoting metastasis. Conversely, reduced stathmin levels correlate with neurodegenerative disorders, as microtubule stability is crucial for neuronal integrity.
Recombinant stathmin proteins are produced via heterologous expression systems (e.g., *E. coli* or mammalian cells) for structural and functional studies. These proteins enable researchers to dissect stathmin-tubulin interactions, phosphorylation effects, and mechanisms underlying microtubule regulation. Mutant variants, mimicking phosphorylation states, help elucidate how post-translational modifications alter stathmin’s activity. Additionally, recombinant stathmin serves as a tool for drug screening, aiming to develop inhibitors that target its microtubule-disrupting function in cancer therapy.
In summary, stathmin recombinant protein is a vital resource for exploring cellular dynamics, disease mechanisms, and therapeutic strategies tied to microtubule modulation.
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