| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | H2A.1; H2A.2; H2A/a; H2A/m; H2A/q; H2A1B; H2AFA; H2AFE; H2AFL; H2AFM; H2AFO; H2AFQ; HIST1H2AE; HIST1H2AJ; HIST2H2AA; HIST2H2AA3; HIST2H2AB; HIST2H2AC;;MonoMethyl-Histone H2A (K118) |
| WB Predicted band size | 14 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human Histone H2A around the methylation site of K118 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于Histone H2A(monomethylK118)抗体的参考文献示例(内容基于研究领域常见主题构造，建议通过学术数据库核实具体文献)：
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1. **文献名称**：*Histone H2A monomethylation at lysine 118 regulates DNA damage response*
**作者**：Smith J. et al.
**摘要**：该研究通过ChIP和免疫荧光技术，发现H2AK118me1在DNA双链断裂位点富集，并证明其通过招募BRCA1复合体参与同源重组修复。
2. **文献名称**：*Epigenetic silencing mediated by H2AK118me1 in heterochromatin formation*
**作者**：Li Y. et al.
**摘要**：利用特异性抗体进行基因组定位分析，揭示H2AK118me1在异染色质区域富集，与HP1蛋白相互作用，调控基因沉默和染色质压缩。
3. **文献名称**：*Development and validation of a monoclonal antibody for histone H2A monomethylated at K118*
**作者**：Wang Q. et al.
**摘要**：报道了H2AK118me1抗体的制备与验证，通过肽阵列和质谱确认其特异性，并应用于癌症细胞系中研究该修饰与肿瘤转移的相关性。
4. **文献名称**：*H2AK118me1 dynamics during embryonic stem cell differentiation*
**作者**：Garcia M. et al.
**摘要**：使用该抗体分析胚胎干细胞分化过程中H2AK118me1的全局变化，发现其水平下降与多能性基因(如Oct4)的沉默密切相关。
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**建议**：通过PubMed或Google Scholar搜索关键词“H2AK118me1”或“Histone H2A monomethylation K118”获取最新文献。若研究较少，可扩展至H2A甲基化的其他位点(如H2AK119)或组蛋白修饰的通用机制。
The histone H2A monomethyllysine 118 (H2A monomethylK118) antibody is a specialized tool used to study post-translational modifications of histone H2A, a core component of nucleosomes. Histone H2A plays a critical role in chromatin structure and gene regulation, and its lysine residues undergo various modifications, including methylation, which influence epigenetic signaling. Monomethylation at lysine 118 (K118) is a specific epigenetic mark associated with transcriptional regulation, DNA repair, and chromatin remodeling. This modification is dynamically regulated by histone methyltransferases and demethylases, contributing to the activation or repression of target genes depending on cellular context.
The H2A monomethylK118 antibody enables the detection and localization of this modification in cells or tissues using techniques like chromatin immunoprecipitation (ChIP), immunofluorescence, or Western blotting. Researchers employ it to investigate its role in processes such as cell differentiation, cancer progression (where aberrant histone methylation is common), or responses to DNA damage. Specificity validation, including knockout controls or peptide competition assays, is essential to confirm its reliability, as cross-reactivity with similar histone marks can occur. Understanding H2A monomethylK118 dynamics provides insights into epigenetic mechanisms underlying development, disease, and potential therapeutic targets.
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