| WB | 咨询技术 | Human,Mouse,Rat |
| IF | ChIP:1/20-1/50 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Histone H3.1, Histone H3, HIST1H3A;;DiMethyl-Histone H3 (K80) |
| WB Predicted band size | 15 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human Histone H3.1 around the methylation site of K80 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于Histone H3(dimethylK79)抗体的3篇参考文献及其摘要要点：
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1. **文献名称**：*DOT1L-mediated H3K79 methylation in chromatin is dispensable for Wnt signaling*
**作者**：Steger DJ et al.
**摘要**：研究利用H3K79me2特异性抗体，通过ChIP-seq分析发现H3K79二甲基化广泛分布于活跃转录基因区域，但其在Wnt信号通路中的功能可能被冗余机制补偿，并非必需。
2. **文献名称**：*MLL-rearranged leukemia is dependent on H3K79 methylation by DOT1L*
**作者**：Okada Y et al.
**摘要**：通过H3K79me2抗体检测，揭示DOT1L介导的H3K79甲基化在MLL基因重排白血病中的关键作用，抑制该修饰可选择性阻断白血病细胞增殖。
3. **文献名称**：*Histone H3K79 methylation patterns in human cells correlate with DNA replication timing*
**作者**：Barski A et al.
**摘要**：利用H3K79me2抗体的全基因组分析表明，该修饰富集于早期复制区域，与转录活性和DNA复制时序调控相关，提示其在染色质动态中的作用。
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以上研究均通过H3K79me2特异性抗体揭示其在基因调控、疾病机制及表观遗传动力学中的功能。如需具体实验细节，建议通过PubMed或期刊官网检索原文。
The Histone H3 (dimethylK79) antibody is a specialized tool used to detect the dimethylation of lysine 79 on histone H3 (H3K79me2), a post-translational modification implicated in chromatin regulation and epigenetic signaling. Histone H3 is a core component of nucleosomes, and its methylation at specific residues influences DNA accessibility, transcription, and genome stability. H3K79 methylation occurs within the globular domain of the histone, a region less commonly modified compared to the N-terminal tail. This modification is catalyzed by the methyltransferase DOT1L (Disruptor of Telomeric Silencing 1-like) in mammals, which exclusively targets H3K79 in a methylation gradient (mono-, di-, or trimethylation) depending on cell cycle phase and genomic context.
H3K79me2 plays roles in transcriptional elongation, DNA damage repair, and cell cycle regulation. It is enriched at actively transcribed gene bodies and interacts with proteins involved in chromatin remodeling and mRNA processing. Aberrant H3K79 methylation has been linked to leukemogenesis, particularly in mixed-lineage leukemia (MLL) rearrangements, where DOT1L activity is dysregulated. The Histone H3 (dimethylK79) antibody is widely used in chromatin immunoprecipitation (ChIP), immunofluorescence, and Western blotting to study its spatial and temporal distribution. Specificity validation via knockout/knockdown controls is critical, as cross-reactivity with other methylated histone residues or isoforms can occur. This antibody serves as a key reagent in epigenetics research, helping to unravel mechanisms of gene expression control and disease-associated chromatin states.
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