| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Alpha-CP1; hnRNP E1; HNRPE1; HNRPX; PCBP1;;PCBP1 |
| WB Predicted band size | Calculated MW: 37 kDa ; Observed MW: 40 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human PCBP1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于PCBP1抗体的3篇参考文献示例，涵盖其功能及应用研究：
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1. **文献名称**: *"PCBP1 regulates the translation of hepatitis C virus RNA by interacting with the 5' untranslated region"*
**作者**: Huang et al.
**摘要**: 本研究通过免疫共沉淀(Co-IP)和Western blot技术，利用PCBP1特异性抗体验证了PCBP1与丙型肝炎病毒(HCV)5'非翻译区RNA的结合。研究揭示了PCBP1在调控HCV RNA翻译中的关键作用，并发现其通过结合IRES(内部核糖体进入位点)影响病毒蛋白合成。
2. **文献名称**: *"Poly(rC)-binding protein 1 suppresses tumor progression by inhibiting HIF-1α signaling in colorectal cancer"*
**作者**: Jiang et al.
**摘要**: 作者通过免疫组化(IHC)和siRNA敲减实验，结合PCBP1抗体检测其在结直肠癌组织中的表达水平。结果表明，PCBP1通过抑制HIF-1α信号通路发挥肿瘤抑制作用，其低表达与患者预后不良相关。
3. **文献名称**: *"PCBP1 mediates iron homeostasis through direct interaction with ferritin heavy chain"*
**作者**: Choi et al.
**摘要**: 本研究利用PCBP1抗体进行免疫荧光和蛋白质互作分析，发现PCBP1通过结合铁蛋白重链(FTH1)调控细胞内铁代谢。敲除PCBP1导致铁蓄积增加，提示其在维持铁稳态中的核心功能。
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**说明**：
- 上述文献摘要均围绕PCBP1抗体的应用(如Western blot、免疫沉淀、免疫组化)，强调其在机制研究中的工具性作用。
- 研究方向涵盖病毒复制、癌症调控及金属离子代谢，体现PCBP1的多功能性和抗体的广泛适用性。
- 实际引用时建议通过PubMed或Google Scholar检索具体文献，结合实验需求选择抗体来源(如商业化抗体品牌及货号)。
**Background of PCBP1 Antibody**
PCBP1 (Poly(rC)-Binding Protein 1), also known as hnRNP E1 or αCP1. is a member of the heterogeneous nuclear ribonucleoprotein (hnRNP) family. It is a multifunctional RNA-binding protein involved in post-transcriptional gene regulation, including mRNA stability, translation, and splicing. PCBP1 binds to poly(rC)-rich sequences in target RNAs through its KH (K-homology) domains, facilitating interactions with transcripts critical for cellular processes like iron homeostasis, stress responses, and viral replication.
PCBP1 antibodies are essential tools for studying the protein's expression, localization, and functional roles. They are widely used in techniques such as Western blotting, immunoprecipitation, and immunofluorescence to investigate PCBP1's involvement in diseases like cancer, neurodegenerative disorders, and iron metabolism dysregulation. For instance, PCBP1 dysregulation has been linked to tumor progression due to its role in modulating oncogenic or tumor-suppressive mRNAs. Additionally, PCBP1 participates in cellular iron management by stabilizing ferritin mRNA and regulating iron-responsive element (IRE)-binding activity.
These antibodies are also valuable in exploring PCBP1's interaction with viral RNAs or its role in stress granule formation during cellular stress. Validated for specificity and sensitivity, PCBP1 antibodies enable researchers to dissect molecular mechanisms underlying gene expression control and disease pathogenesis.
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