| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Histone H3.1, Histone H3, HIST1H3A;;Symmetric DiMethyl-Histone H3 (R17) |
| WB Predicted band size | 15 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | A synthesized peptide derived from human Histone H3.1 around the methylation site of R17 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于Histone H3对称二甲基化R17(H3R17me2s)抗体的3篇参考文献，简要概括如下：
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1. **文献名称**：*PRMT4-mediated symmetric dimethylation of histone H3R17 promotes transcriptional activation*
**作者**：Bauer, U.M. et al.
**摘要**：本研究揭示了PRMT4(CARM1)催化组蛋白H3第17位精氨酸(R17)的对称二甲基化修饰，并证明该修饰与核受体(如雌激素受体)介导的基因转录激活密切相关，为表观遗传调控机制提供了新见解。
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2. **文献名称**：*Histone arginine methylation and its dynamic regulation*
**作者**：Wang, H. et al.
**摘要**：文章系统综述了组蛋白精氨酸甲基化的生物学功能，重点关注H3R17对称二甲基化在基因表达调控中的作用，并讨论了相关抗体在染色质免疫沉淀(ChIP)中的特异性验证与应用。
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3. **文献名称**：*Symmetric dimethylation of H3R17 by PRMT5 is linked to oncogenic gene expression*
**作者**：Pal, S. et al.
**摘要**：研究发现PRMT5介导的H3R17对称二甲基化在多种癌症中异常上调，通过激活促癌基因(如c-Myc)驱动肿瘤进展，并利用特异性抗体验证了该修饰与临床预后的相关性。
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**备注**：H3R17的对称二甲基化相关研究相对较少，部分文献可能涉及不对称修饰(如PRMT4/CARM1催化H3R17不对称二甲基化)。建议结合抗体供应商(如CST、Abcam)的产品说明书引用具体验证数据。
The Histone H3 symmetric dimethylarginine at residue 17 (H3R17me2s) antibody is a critical tool for studying post-translational modifications (PTMs) associated with epigenetic regulation. Histone H3 is a core component of nucleosomes, and arginine methylation at specific residues, such as R17. plays a role in modulating chromatin structure and gene expression. Symmetric dimethylation of H3R17 is catalyzed by protein arginine methyltransferases (PRMTs), particularly PRMT5. which transfers methyl groups to arginine residues in a symmetrical configuration. This modification is linked to transcriptional regulation, DNA repair, and cellular differentiation.
H3R17me2s is implicated in both gene activation and repression, depending on the genomic context and interacting proteins. It has been associated with maintaining heterochromatin stability and recruiting effector proteins that mediate downstream signaling. Dysregulation of H3R17 methylation is observed in cancers, developmental disorders, and other diseases, making this modification a potential therapeutic target.
The H3R17me2s antibody enables researchers to detect and quantify this PTM using techniques like chromatin immunoprecipitation (ChIP), immunofluorescence, and Western blotting. Specificity validation is crucial, often involving knockout cells or peptide competition assays to ensure the antibody does not cross-react with similar epitopes (e.g., asymmetric dimethylarginine or other methylated histone residues). Its application advances studies in epigenetics, cancer biology, and stem cell research, providing insights into how arginine methylation fine-tunes cellular processes.
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