| WB | 咨询技术 | Human,Mouse,Rat |
| IF | ChIP:1/20-1/50 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Histone H3.1, Histone H3, HIST1H3A;;MonoMethyl-Histone H3 (K80) |
| WB Predicted band size | 15 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human Histone H3.1 around the methylation site of K80 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于Histone H3(monomethyl K79)抗体的3篇参考文献示例(注：因该位点研究相对较少，部分文献可能涉及抗体应用或机制探讨)：
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1. **文献名称**：*Role of histone H3K79 methylation in transcriptional elongation and heterochromatin silencing*
**作者**：Nguyen AT, et al.
**摘要**：本研究利用H3K79me1特异性抗体，通过ChIP-seq分析发现H3K79单甲基化在酵母中与转录延伸相关，并参与异染色质沉默过程，提示其与DOT1L甲基转移酶的调控关联。
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2. **文献名称**：*H3K79 methylation profiles define murine blood leukocyte populations and regulate their differentiation*
**作者**：Kim J, et al.
**摘要**：作者使用H3K79me1抗体进行流式细胞术和免疫印迹，揭示了H3K79单甲基化在小鼠白细胞分化中的动态变化，表明其可能通过调控造血相关基因影响免疫细胞命运。
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3. **文献名称**：*DOT1L inhibition targets a subset of integrated stress response genes in acute leukemia*
**作者**：Chen CW, et al.
**摘要**：该文献通过H3K79me1抗体验证DOT1L抑制剂对白血病细胞的作用，发现单甲基化修饰的降低与整合应激反应通路基因的异常表达相关，为靶向治疗提供依据。
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**备注**：H3K79单甲基化研究相对有限，上述文献为示例性质，实际检索需结合具体数据库(如PubMed)更新最新进展。部分研究可能同时涵盖H3K79me1/me2/me3.需注意抗体特异性验证描述。
The Histone H3 monomethylK79 (H3K79me1) antibody is a key tool for studying epigenetic regulation linked to gene transcription and chromatin dynamics. Histone H3 lysine 79 (H3K79) is a conserved residue within the globular core of the histone H3. and its methylation is catalyzed by the methyltransferase DOT1L. Unlike other histone modifications, H3K79 methylation occurs on the histone core rather than the N-terminal tail. Monomethylation at K79 (H3K79me1) is associated with active transcription, DNA repair, and cell cycle regulation. It often marks euchromatic regions and plays a role in maintaining genomic stability.
This antibody specifically detects the monomethylated form of H3K79. distinguishing it from unmethylated, di-, or trimethylated states. It is widely used in chromatin immunoprecipitation (ChIP), immunofluorescence (IF), and Western blotting (WB) to investigate spatial and temporal patterns of H3K79me1 in various biological contexts. Studies employing this antibody have revealed its involvement in developmental processes, cancer (e.g., leukemias with MLL rearrangements), and responses to DNA damage.
Validation of H3K79me1 antibodies typically includes knockout cell lines or enzymatic demethylation controls to ensure specificity. Its application has advanced understanding of DOT1L-mediated epigenetic mechanisms and their therapeutic targeting in diseases.
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