| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | STAP1 |
| Uniprot No | Q9ULZ2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-295aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSHMMAKKP PKPAPRRIFQ ERLKITALPL YFEGFLLIKR SGYREYEHYW TELRGTTLFF YTDKKSIIYV DKLDIVDLTC LTEQNSTEKN CAKFTLVLPK EEVQLKTENT ESGEEWRGFI LTVTELSVPQ NVSLLPGQVI KLHEVLEREK KRRIETEQST SVEKEKEPTE DYVDVLNPMP ACFYTVSRKE ATEMLQKNPS LGNMILRPGS DSRNYSITIR QEIDIPRIKH YKVMSVGQNY TIELEKPVTL PNLFSVIDYF VKETRGNLRP FICSTDENTG QEPSMEGRSE KLKKNPHIA |
| 预测分子量 | 37 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于STAP1重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**: *STAP1 mutations in hypercholesterolemic patients indicate a role for STAP1 in lipid metabolism*
**作者**: Huijgen R, et al.
**摘要**: 本研究通过基因测鉴发现STAP1基因突变与家族性高胆固醇血症相关。作者通过体外表达重组STAP1蛋白，证明其参与调节LDL受体表达，并影响胆固醇代谢通路，提示STAP1可能在脂质代谢中发挥重要作用。
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2. **文献名称**: *Structural and functional characterization of recombinant STAP1 protein in JAK-STAT signaling*
**作者**: Park JH, et al.
**摘要**: 该研究利用大肠杆菌系统成功表达并纯化STAP1重组蛋白。通过X射线晶体学解析其三维结构，发现STAP1通过SH2结构域与JAK激酶相互作用，调控STAT蛋白磷酸化，揭示了其在JAK-STAT信号通路中的分子机制。
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3. **文献名称**: *Recombinant STAP1 protein enhances adipogenesis through modulation of PPARγ activity*
**作者**: Li Y, et al.
**摘要**: 文章报道了STAP1重组蛋白在3T3-L1前脂肪细胞分化中的作用。实验表明，STAP1通过结合PPARγ转录因子促进脂肪生成相关基因表达，为研究肥胖和代谢综合征提供了新的分子靶点。
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注：以上文献为模拟示例，实际研究中建议通过PubMed或Web of Science以“STAP1 recombinant protein”等关键词检索最新文献。部分早期研究可能聚焦于STAP1基因功能，需进一步筛选涉及重组蛋白制备与应用的内容。
**Background of STAP1 Recombinant Protein**
STAP1 (Signal-transducing adaptor protein 1) is a cytosolic adaptor protein involved in regulating intracellular signaling pathways, particularly those linked to immune responses and cellular proliferation. Initially identified for its role in B-cell receptor signaling, STAP1 contains modular domains such as a pleckstrin homology (PH) domain and a Src homology 2 (SH2) domain, enabling interactions with phosphorylated tyrosine residues and membrane phospholipids. These structural features allow it to act as a scaffold, facilitating signal transduction between membrane receptors and downstream effectors.
Studies implicate STAP1 in pathways like JAK-STAT, NF-κB, and PI3K-AKT, influencing immune cell activation, apoptosis, and metabolic regulation. Genetic variants in *STAP1* have been associated with autoimmune disorders, hypercholesterolemia, and certain cancers, highlighting its clinical relevance. For instance, gain-of-function mutations may drive aberrant B-cell signaling in lymphomas, while loss-of-function variants correlate with metabolic dysregulation.
Recombinant STAP1 protein is engineered using expression systems (e.g., *E. coli* or mammalian cells) to produce purified, functional protein for mechanistic studies. Its production enables researchers to investigate protein-protein interactions, kinase activities, and structural dynamics in vitro. Additionally, recombinant STAP1 serves as a tool for developing targeted therapies or diagnostic biomarkers, given its disease associations. Recent research also explores its role in T-cell tolerance and adipose tissue inflammation, broadening its potential therapeutic applications.
Despite progress, STAP1's full interactome and context-dependent signaling roles remain under investigation, necessitating further studies to unravel its dual roles in health and disease.
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