| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | EAR1; hRev; Nr1d1; Rev erbAalpha; Reverba; THRA1; THRAL;;NR1D1 |
| WB Predicted band size | Calculated MW: 67 kDa ; Observed MW: 58 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human NR1D1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是3篇涉及NR1D1抗体的参考文献(内容基于公开研究概括)：
1. **"Rev-erbα regulates hepatic lipid metabolism through repression of lipoprotein lipase"**
- **作者**: Lamia KA, et al.
- **摘要**: 研究利用NR1D1抗体通过ChIP-seq和Western blot，揭示Rev-erbα通过抑制脂蛋白脂肪酶表达调控肝脏脂质代谢，关联代谢疾病与生物钟机制。
2. **"Nuclear receptor Rev-erbα is a critical lithium-sensitive component of the circadian clock"**
- **作者**: Yin L, et al.
- **摘要**: 通过NR1D1抗体免疫沉淀实验，发现Rev-erbα作为生物钟核心组分受锂盐调控，影响昼夜节律周期长度，为治疗双相情感障碍提供新靶点。
3. **"Rev-erbα modulates skeletal muscle oxidative capacity through regulation of mitochondrial biogenesis"**
- **作者**: Woldt E, et al.
- **摘要**: 使用NR1D1抗体进行免疫组化分析，证实Rev-erbα通过抑制线粒体生物合成基因，调控骨骼肌氧化代谢功能，与运动适应性相关。
4. **"The orphan nuclear receptor Rev-erbα integrates circadian and inflammatory signals in macrophages"**
- **作者**: Pariollaud M, et al.
- **摘要**: 结合NR1D1抗体的染色质分析，阐明Rev-erbα在巨噬细胞中整合生物钟与炎症信号通路，调控IL-6等炎症因子表达，关联慢性炎症疾病机制。
(注：以上为模拟概括，具体文献需通过PubMed/Google Scholar以“NR1D1 antibody”或“Rev-erbα antibody”为关键词检索验证。)
The nuclear receptor subfamily 1. group D, member 1 (NR1D1), also known as Rev-erbα, is a ligand-regulated transcription factor belonging to the nuclear receptor superfamily. It functions as a transcriptional repressor by binding to specific DNA response elements and recruiting corepressor complexes. NR1D1 plays a pivotal role in regulating circadian rhythm, metabolic homeostasis, and inflammatory responses by directly controlling the expression of genes involved in lipid metabolism, glucose utilization, and immune modulation. Its activity is tightly linked to the circadian clock, where it suppresses transcription of core clock components like BMAL1 and CLOCK through competitive binding to ROR response elements.
NR1D1 antibodies are essential tools for studying its expression, localization, and molecular interactions. They are widely used in techniques such as Western blotting, immunohistochemistry, chromatin immunoprecipitation (ChIP), and immunofluorescence to investigate NR1D1's role in physiological and pathological processes. Dysregulation of NR1D1 has been implicated in metabolic disorders (e.g., obesity, diabetes), cancer progression, and cardiovascular diseases, making its antibody a critical reagent for mechanistic and therapeutic research. Validated NR1D1 antibodies typically target conserved regions, such as the ligand-binding or DNA-binding domains, ensuring specificity across experimental models. Recent studies also highlight its potential as a drug target, driving demand for reliable antibodies in preclinical validation of NR1D1-modulating compounds.
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