| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | GCK; GK; GLK; Glucokinase; HHF3; HK IV; HK4; HKIV; HXKP; LGLK; MODY2;;GCK |
| WB Predicted band size | 52 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human GCK |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
+ +
以下是关于Glucokinase抗体的3篇参考文献示例(内容为虚构，仅供格式参考)：
1. **文献名称**: "Development of a Monoclonal Antibody Specific for Human Glucokinase and Its Application in Hepatic Tissue Analysis"
**作者**: Tanaka A, et al.
**摘要**: 研究报道了一种高特异性单克隆抗体的制备，通过免疫印迹和免疫组化验证其在人肝组织中对Glucokinase的特异性检测，用于研究糖尿病中肝糖代谢异常。
2. **文献名称**: "Characterization of Glucokinase Isoforms Using Polyclonal Antibodies in Pancreatic β-Cells"
**作者**: Smith JL, et al.
**摘要**: 利用兔源多克隆抗体区分Glucokinase的不同剪切变体，揭示了β细胞中Glucokinase亚型的表达差异及其在胰岛素分泌调控中的作用。
3. **文献名称**: "A Novel Glucokinase Antibody-Based ELISA for Early Diagnosis of MODY2"
**作者**: Chen H, et al.
**摘要**: 开发了一种基于Glucokinase抗体的ELISA检测方法，用于筛查青少年起病的成人型糖尿病(MODY2)患者中Glucokinase基因突变导致的蛋白表达异常。
如需实际文献，建议在PubMed或Web of Science中检索关键词“Glucokinase antibody”，并筛选近年高被引研究。
Glucokinase (GK), also known as hexokinase IV, is a critical enzyme in glucose homeostasis, primarily expressed in pancreatic β-cells and hepatocytes. It catalyzes the phosphorylation of glucose to glucose-6-phosphate, serving as a glucose sensor to regulate insulin secretion and hepatic glucose metabolism. Antibodies targeting glucokinase are essential tools for studying its expression, localization, and function in metabolic tissues. These antibodies are widely used in techniques such as Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to investigate GK's role in diabetes, particularly in maturity-onset diabetes of the young type 2 (MODY2), linked to GK gene mutations.
Glucokinase antibodies are typically raised against specific epitopes of the enzyme, often derived from recombinant human or rodent GK proteins. They enable researchers to assess tissue-specific GK expression levels, which can vary under different metabolic conditions, such as fasting or hyperglycemia. Validation of these antibodies includes testing in knockout models or siRNA-treated cells to confirm specificity. Commercially available GK antibodies may differ in clonality (monoclonal or polyclonal), species reactivity, and applications, requiring careful selection based on experimental needs. Their use has advanced understanding of GK's regulatory mechanisms and potential therapeutic targets for diabetes and metabolic disorders.
×
