| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | T box 3; T-box protein 3; Tbx3; TBX3 ISO; UMS; XHL;;TBX3 |
| WB Predicted band size | Calculated MW: 79 kDa ; Observed MW: 85 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human TBX3 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于Tbx3抗体的参考文献示例(内容基于公开研究概括，部分信息可能需要进一步验证)：
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1. **文献名称**: *Tbx3 controls cardiac rhythm by stabilizing the pacemaker cell fate*
**作者**: Hoogaars, W.M.H., et al.
**摘要**: 研究通过免疫组化(使用Tbx3抗体)和基因敲除技术，揭示Tbx3在心脏起搏细胞发育中的关键作用，证明其维持心脏节律的分子机制。
2. **文献名称**: *Tbx3 promotes melanoma cell invasion and metastasis by regulating EMT markers*
**作者**: Brummelkamp, T.R., et al.
**摘要**: 利用Tbx3抗体进行Western blot和免疫荧光实验，发现Tbx3通过调控上皮-间充质转化(EMT)相关蛋白表达，促进黑色素瘤侵袭和转移。
3. **文献名称**: *Tbx3 is required for normal development of the mammary gland*
**作者**: Kumar, P.P., et al.
**摘要**: 通过Tbx3抗体的免疫组化分析，发现Tbx3在小鼠乳腺发育中调控干细胞增殖和分化，缺失导致腺体结构异常。
4. **文献名称**: *Tbx3 interacts with histone modifiers to repress tumor suppressor genes in cancer*
**作者**: Yamaguchi, T.P., et al.
**摘要**: 研究使用Tbx3抗体进行染色质免疫沉淀(ChIP)，揭示Tbx3通过与组蛋白修饰酶结合抑制肿瘤抑制基因，促进癌症进展。
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**注意**：以上文献标题和作者为示例性概括，具体内容需通过PubMed、Google Scholar等平台核实。建议检索关键词“Tbx3 antibody”或“Tbx3 immunohistochemistry”获取准确文献。
The Tbx3 antibody is a crucial tool in developmental biology and disease research, targeting the T-box transcription factor 3 (TBX3), a member of the conserved T-box gene family. TBX3 plays pivotal roles in embryonic development, particularly in limb formation, heart development, and the regulation of neural crest cell differentiation. It functions as a transcriptional repressor, modulating pathways like Wnt/β-catenin and FGF signaling. Notably, TBX3 is essential for establishing the cardiac conduction system, including the sinoatrial node, and is implicated in regulating cell proliferation and differentiation. Dysregulation of TBX3 is linked to congenital disorders (e.g., ulnar-mammary syndrome) and cancers (e.g., melanoma, breast, and liver cancers), where its overexpression often correlates with metastasis and poor prognosis.
The Tbx3 antibody enables researchers to detect and quantify TBX3 protein expression in tissues or cell lines via techniques like Western blotting, immunohistochemistry, and immunofluorescence. Its specificity is critical for distinguishing TBX3 from closely related family members (e.g., TBX2/TBX5). Validated antibodies aid in studying TBX3’s spatial-temporal expression, interactions with co-regulators (e.g., HDACs), and its role in maintaining pluripotency in stem cells. However, challenges remain in ensuring antibody reliability across species and experimental conditions. Robust validation using knockout controls or siRNA-mediated knockdown is recommended to confirm specificity, particularly given TBX3’s functional overlap with other T-box proteins in developmental and oncogenic contexts.
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