| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | STAMBP |
| Uniprot No | O95630 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-424aa |
| 氨基酸序列 | MSDHGDVSLPPEDRVRALSQLGSAVEVNEDIPPRRYFRSGVEIIRMASIY SEEGNIEHAFILYNKYITLFIEKLPKHRDYKSAVIPEKKDTVKKLKEIAF PKAEELKAELLKRYTKEYTEYNEEKKKEAEELARNMAIQQELEKEKQRVA QQKQQQLEQEQFHAFEEMIRNQELEKERLKIVQEFGKVDPGLGGPLVPDL EKPSLDVFPTLTVSSIQPSDCHTTVRPAKPPVVDRSLKPGALSNSESIPT IDGLRHVVVPGRLCPQFLQLASANTARGVETCGILCGKLMRNEFTITHVL IPKQSAGSDYCNTENEEELFLIQDQQGLITLGWIHTHPTQTAFLSSVDLH THCSYQMMLPESVAIVCSPKFQETGFFKLTDHGLEEISSCRQKGFHPHSK DPPLFCSCSHVTVVDRAVTITDLR |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于STAMBP重组蛋白的3篇代表性文献摘要，供参考：
---
1. **文献名称**: *STAMBP/AMSH regulates EGFR trafficking via deubiquitination in endosomes*
**作者**: Kato M. et al.
**摘要**: 本研究通过在大肠杆菌中重组表达人源STAMBP蛋白，验证其作为去泛素化酶(DUB)的活性。实验表明，STAMBP通过特异性切割K63-linked泛素链，调控表皮生长因子受体(EGFR)的内吞分选，从而影响下游信号传导。
2. **文献名称**: *Structural basis for STAMBP-mediated ubiquitin chain cleavage*
**作者**: McCullough J. et al.
**摘要**: 利用昆虫细胞系统表达并纯化重组STAMBP蛋白，结合X射线晶体学解析其三维结构。研究发现，STAMBP通过保守的JAMM结构域选择性识别泛素链，揭示了其底物特异性的分子机制。
3. **文献名称**: *Functional analysis of STAMBP mutations in microcephaly-capillary malformation syndrome*
**作者**: Muhle H. et al.
**摘要**: 构建野生型和突变型STAMBP重组蛋白，发现致病突变导致其去泛素化酶活性丧失，并破坏与STAM1的相互作用，阐明了该蛋白在神经发育中的关键作用。
---
**注**：以上文献信息为示例性概括，实际研究中请通过PubMed或Web of Science以关键词“STAMBP recombinant”“STAMBP deubiquitinase”等检索最新原文。
**Background of STAMBP Recombinant Protein**
STAMBP (Signal Transducing Adaptor Molecule-Binding Protein), also known as AMSH (Associated Molecule with the SH3 Domain of STAM), is a deubiquitinating enzyme (DUB) involved in critical cellular processes, including endosomal sorting, signal transduction, and protein trafficking. It belongs to the JAMM/MPN+ metalloprotease family and specifically cleaves Lys63-linked polyubiquitin chains, a modification often associated with endocytic sorting and DNA repair. STAMBP interacts with components of the ESCRT (Endosomal Sorting Complex Required for Transport) machinery, regulating the sorting of ubiquitinated cargo proteins into multivesicular bodies (MVBs) for lysosomal degradation or extracellular secretion.
Biologically, STAMBP modulates receptor tyrosine kinase (RTK) signaling by controlling the stability and trafficking of activated receptors, such as EGFR and c-MET. Dysregulation of STAMBP is linked to neurodevelopmental disorders, including microcephaly-capillary malformation syndrome (MIC-CAP), caused by mutations that impair its catalytic activity or cellular localization. These mutations disrupt neural cell proliferation, migration, and survival, highlighting its role in brain development.
Recombinant STAMBP proteins are engineered in vitro using expression systems like *E. coli* or mammalian cells, often fused with tags (e.g., His-tag) for purification and detection. These proteins serve as tools to study enzymatic mechanisms, substrate specificity, and interactions with ESCRT components. Additionally, they are used in drug discovery to screen DUB inhibitors or validate pathogenic mutations. Research on STAMBP recombinant proteins enhances understanding of intracellular trafficking pathologies and potential therapeutic strategies for related genetic disorders.
In summary, STAMBP is a pivotal enzyme in ubiquitin-dependent signaling and trafficking, with recombinant forms enabling mechanistic and translational studies in cell biology and disease.
×
