| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | END1; hVPS11; PEP5; PP3476; RNF108; vps11;;VPS11 |
| WB Predicted band size | 108 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | A synthesized peptide derived from human VPS11 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于VPS11抗体的3篇参考文献的简要信息：
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1. **文献名称**：*VPS11 regulates autophagy via modulating lysosomal degradation in mammalian cells*
**作者**：Li X. et al.
**摘要**：本研究利用VPS11特异性抗体，通过免疫印迹和免疫荧光技术，揭示VPS11通过与HOPS复合体相互作用调控自噬体-溶酶体融合，其缺失导致溶酶体功能异常和自噬底物积累，提示其在神经退行性疾病中的潜在作用。
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2. **文献名称**：*Role of VPS11 in the biogenesis of lysosome-related organelles*
**作者**：Zhang Y. et al.
**摘要**：作者通过CRISPR敲除结合VPS11抗体验证，发现VPS11在黑色素细胞中参与溶酶体相关细胞器(LROs)的形成，其缺失导致黑素体成熟缺陷，为遗传性色素疾病提供了分子机制解释。
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3. **文献名称**：*VPS11 variants associated with Parkinson's disease impair vesicular trafficking*
**作者**：Wang R. et al.
**摘要**：该研究在帕金森病患者中发现VPS11基因突变，利用抗体进行蛋白表达分析，显示突变体导致内体-溶酶体运输障碍，α-突触核蛋白清除受阻，为VPS11在神经病理中的功能提供了直接证据。
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**备注**：上述文献为虚拟示例，实际引用时请通过PubMed或Google Scholar检索真实文献(关键词：VPS11 antibody, HOPS complex, lysosomal disorders)。近年研究多聚焦于VPS11在溶酶体疾病及神经退行性病变中的机制。
The VPS11 antibody is a research tool used to study the Vacuolar Protein Sorting 11 (VPS11) protein, a key component of cellular trafficking pathways. VPS11 is part of the HOPS (Homotypic Fusion and Protein Sorting) complex, which mediates lysosome biogenesis, endosome-lysosome fusion, and autophagosome maturation. It plays a critical role in regulating vesicular transport, membrane fusion, and organelle acidification. Dysfunction of VPS11 is linked to lysosomal storage disorders, neurodegenerative diseases, and cancer.
The antibody specifically targets VPS11. enabling detection and localization of the protein in cells or tissues. It is widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to investigate VPS11 expression, interaction partners, and cellular distribution. Researchers utilize this tool to explore mechanisms underlying lysosomal defects, autophagy impairment, or abnormal protein aggregation in diseases like Alzheimer's or Parkinson's.
Commercial VPS11 antibodies are typically developed in hosts like rabbits or mice, validated for specificity using knockout controls. Its applications span basic research in cell biology, drug discovery targeting lysosomal pathways, and diagnostic studies of genetic disorders. However, optimal performance may vary depending on experimental conditions, requiring careful optimization. Overall, the VPS11 antibody is essential for deciphering the protein's role in cellular homeostasis and disease pathology.
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