| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CSE1L; CAS; CSE1; Exp2; Importin-alpha re-exporter; Exportin-2; XPO2;;Exportin 2 |
| WB Predicted band size | Calculated MW: 110 kDa ; Observed MW: 98 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | A synthesized peptide derived from human Exportin 2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于Exportin2(XPO2/CSE1L)抗体的3篇参考文献，内容涵盖功能研究和应用方向：
1. **"CSE1L/CAS: its role in proliferation, apoptosis, and cancer"**
*作者：B. Brinkmann 等*
摘要：该综述总结了Exportin2(CSE1L)在核质运输、细胞增殖和凋亡中的作用，并讨论了其抗体在癌症研究中的应用，如通过免疫组化检测肿瘤组织中CSE1L表达水平与预后的关联。
2. **"Structural insights into the role of Exportin-2 in snRNA export"**
*作者：A. D. Moen 等*
摘要：研究通过冷冻电镜解析Exportin2与snRNA复合物的结构，揭示了其运输机制。文中提到使用Exportin2特异性抗体进行免疫共沉淀(Co-IP)验证关键蛋白相互作用。
3. **"XPO2-mediated nuclear export of 60S ribosomal subunits is required for protein synthesis"**
*作者：L. Li 等*
摘要：该研究阐明Exportin2参与核糖体亚基转运的功能，通过siRNA敲低和抗体阻断实验证明其缺失会导致蛋白质合成受阻，为靶向XPO2的抗体工具在分子机制研究中的应用提供依据。
注：实际文献检索建议通过PubMed或Google Scholar结合关键词(Exportin2/XPO2/CSE1L + antibody/function)获取最新研究。
Exportin 2. also known as CSE1L or chromosomal segregation 1-like protein, is a member of the importin-β family of nuclear transport receptors. It plays a critical role in nucleocytoplasmic shuttling, particularly in the recycling of importin-α from the nucleus to the cytoplasm after nuclear import processes. Exportin 2 binds to importin-α in a RanGTP-dependent manner, facilitating its re-export to the cytoplasm for subsequent rounds of nuclear transport. Beyond its transport functions, Exportin 2 has been implicated in mitotic spindle assembly, cell cycle regulation, and signal transduction pathways.
Antibodies targeting Exportin 2 are widely used tools in molecular and cell biology research. These antibodies enable the detection and localization of Exportin 2 in various experimental applications, including Western blotting, immunofluorescence, and immunohistochemistry. They are particularly valuable for studying nuclear transport mechanisms, cellular compartmentalization dynamics, and protein-protein interactions involving transport receptors. Commercially available Exportin 2 antibodies are typically developed using immunogens corresponding to specific epitopes within the human CSE1L protein sequence.
Research utilizing Exportin 2 antibodies has revealed its potential involvement in cancer progression, with studies showing altered expression levels in colorectal, breast, and hepatocellular carcinomas. These antibodies also contribute to investigations of cellular stress responses, as Exportin 2 appears to interact with proteins involved in apoptosis and DNA damage repair pathways. Validation of Exportin 2 antibodies generally includes knockout cell line controls to confirm specificity, given the protein's structural complexity and functional diversity.
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