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Rabbit Monoclonal Presenilin1 Antibody

  • 中文名： Presenilin1抗体
  • 别    名： AD3; Ad3h; FAD; Protein S182; PS1-CTF12; PSEN1; PSNL1; S182;;Presenilin 1
货号: IPDX17993
Price： ￥1280
数量：
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验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 咨询技术 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 1/20-1/100 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesAD3; Ad3h; FAD; Protein S182; PS1-CTF12; PSEN1; PSNL1; S182;;Presenilin 1
WB Predicted band sizeCalculated MW: 53 kDa ; Observed MW: 28 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman,Mouse,Rat
ImmunogenA synthesized peptide derived from human Presenilin 1
FormulationPurified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol.

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参考文献

以下是3篇关于Presenilin1(PSEN1)抗体的参考文献及其简要摘要：

1. **"Endoproteolysis of Presenilin 1 and accumulation of processed derivatives in vivo"**

- **作者**: Thinakaran, G. 等(1996)

- **摘要**: 该研究利用特异性PSEN1抗体(针对N端和C端表位)，通过免疫印迹和免疫沉淀技术，首次揭示了PSEN1在体内经历内源性蛋白酶切割，生成稳定的N端和C端片段。抗体验证了PSEN1加工过程与阿尔茨海默病中γ-分泌酶活性的关联。

2. **"Gamma-secretase cleavage of the amyloid precursor protein: Heterogeneity of the proteolytic products and effects of presenilin 1 mutations"**

- **作者**: Duff, K. 等(1996)

- **摘要**: 通过构建PSEN1基因敲除小鼠模型，并采用多种PSEN1抗体进行Western blot分析，研究发现PSEN1缺失导致γ-分泌酶活性丧失，且C92S突变显著增加Aβ42生成，抗体结果支持PSEN1在APP切割中的核心作用。

3. **"Functional gamma-secretase inhibitors reduce beta-amyloid peptide levels in brains of living PS1/APP transgenic mice"**

- **作者**: De Strooper, B. 等(2001)

- **摘要**: 研究通过PSEN1抗体结合免疫组化技术，证实抑制γ-分泌酶可降低转基因小鼠脑内Aβ沉积。抗体检测显示PSEN1在神经元中的定位与γ-分泌酶复合体的分布一致，进一步阐明其在淀粉样斑块形成中的机制。

这些文献均通过特异性抗体解析了PSEN1的蛋白加工、功能及其在阿尔茨海默病病理中的角色。

背景信息

Presenilin 1 (PSEN1) is a critical component of the γ-secretase complex, a multisubunit protease responsible for the intramembrane cleavage of various type I transmembrane proteins, including amyloid precursor protein (APP). Cleavage of APP by γ-secretase generates amyloid-β (Aβ) peptides, particularly Aβ42. which aggregates into plaques in Alzheimer’s disease (AD). Mutations in the PSEN1 gene are the most common cause of early-onset familial AD, highlighting its central role in Aβ pathology. PSEN1 antibodies are essential tools for studying its expression, localization, and function in both physiological and pathological contexts. These antibodies target specific epitopes of the PSEN1 protein, enabling detection in techniques like Western blotting, immunohistochemistry, and immunoprecipitation. Researchers use them to investigate PSEN1 processing (e.g., cleavage into N- and C-terminal fragments), interactions with γ-secretase subunits, and alterations in AD models or patient tissues. Some antibodies distinguish between full-length PSEN1 and its proteolytic fragments, aiding in studies of its maturation and activity. Additionally, PSEN1 antibodies are employed to validate knockout or knockdown models and assess mutant PSEN1 effects on Aβ production. Their applications extend to exploring PSEN1’s non-proteolytic roles in calcium homeostasis, autophagy, and Wnt signaling. As dysregulated PSEN1 contributes broadly to neurodegeneration, these antibodies remain pivotal in advancing AD research and therapeutic development targeting γ-secretase activity.

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