首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人整合素连接激酶1(ILK-1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的ILK含量。ILK（整合素连接激酶）是一种Ser/Thr蛋白激酶，属于TKL家族，主要定位于细胞质和细胞核。它在细胞增殖、迁移和侵袭中发挥重要作用，参与多种信号通路，如PI3K-Akt和Rho/ROCK。ILK的研究机制主要涉及其在细胞骨架重组、细胞迁移和侵袭中的作用，以及与肿瘤发生发展的关系。试剂盒检测范围为39.06 pg/mL-2500 pg/mL，适用于体外培养细胞信号机制研究、疾病模型组织分析或药物干预实验中的生物标志物检测。科研人员可通过该产品探究ILK - 1在肿瘤微环境调控、纤维化疾病进展或心血管重构中的分子机制，为靶向治疗研究提供可靠数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：59 kDa serine/threonine protein kinase ELISA Kit; 59 kDa serine/threonine-protein kinase ELISA Kit; DKFZp686F1765 ELISA Kit; Epididymis secretory protein Li 28 ELISA Kit; HEL S 28 ELISA Kit; ILK 1 ELISA Kit; ILK 2 ELISA Kit; ILK ELISA Kit; ILK-1 ELISA Kit; ILK-2 ELISA Kit; ILK_HUMAN ELISA Kit; ILK1 ELISA Kit; ILK2 ELISA Kit; Integrin linked kinase 2 ELISA Kit; Integrin linked Kinase ELISA Kit; Integrin linked protein kinase ELISA Kit; Integrin-linked protein kinase ELISA Kit; p59 ELISA Kit; p59ILK ELISA Kit
缩写：ILK
Uniprot No.：Q13418
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：39.06 pg/mL-2500 pg/mL
灵敏度：9.77 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays):CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human ILK-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 84 | |||||
| Range % | 80-88 | ||||||
| 1:2 | Average % | 97 | |||||
| Range % | 92-101 | ||||||
| 1:4 | Average % | 90 | |||||
| Range % | 86-94 | ||||||
| 1:8 | Average % | 105 | |||||
| Range % | 100-110 | ||||||
回收率：
| The recovery of human ILK-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 95 | 90-100 | |||||
| EDTA plasma (n=4) | 87 | 83-91 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected25002.0282.1462.0871.94512501.5501.6201.5851.4436251.1071.1681.1380.996312.50.7200.7510.7360.594156.250.4130.4090.4110.26978.130.3000.2860.2930.15139.060.2100.2170.2140.07200.1390.1450.142 | |||||||
货期：3-5 working days
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