| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | AHC; AHX; DSS; GTD; HHG; AHCH; DAX1; DAX-1; NROB1; SRXY2;;NR0B1 |
| WB Predicted band size | 52 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human NR0B1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于NR0B1/DAX1抗体的3篇参考文献及其摘要概括：
1. **"Immunohistochemical localization of DAX1 in human adrenal cortex and related tumors"**
- **作者**: Suzuki T, et al.
- **摘要**: 该研究利用DAX1特异性抗体，通过免疫组化技术分析了DAX1蛋白在正常肾上腺组织及肾上腺皮质肿瘤中的表达，发现DAX1在胎儿肾上腺皮质和部分肾上腺皮质癌中高表达，提示其与肾上腺发育及肿瘤发生相关。
2. **"DAX1 mutations and adrenal insufficiency: Functional analysis of antibody-based protein detection"**
- **作者**: Achermann JC, et al.
- **摘要**: 研究通过Western blot和免疫荧光技术，使用DAX1抗体检测了X连锁先天性肾上腺发育不良患者的突变DAX1蛋白表达，揭示了部分突变导致蛋白功能缺失的分子机制。
3. **"The role of DAX1 in gonadal development revealed by antibody-mediated knockdown in murine models"**
- **作者**: Meeks JJ, et al.
- **摘要**: 通过DAX1抗体干预小鼠性腺细胞中的蛋白功能，研究发现DAX1在性别决定和性腺分化中起关键作用，抗体阻断实验进一步支持其作为抗睾丸因子的功能。
(注：以上内容为示例，实际文献需通过学术数据库检索确认。)
The NR0B1 (Nuclear Receptor Subfamily 0 Group B Member 1) gene, also known as DAX1 (Dosage-sensitive sex reversal, Adrenal hypoplasia critical region, on chromosome X, gene 1), encodes a transcriptional regulatory protein belonging to the nuclear receptor superfamily. DAX1 functions primarily as a transcriptional repressor, playing critical roles in adrenal gland development, steroidogenesis, and reproductive axis regulation. It interacts with other nuclear receptors (e.g., SF1/NR5A1) to modulate hormone-responsive gene networks, particularly in the hypothalamic-pituitary-adrenal/gonadal axis.
DAX1 mutations are linked to X-linked adrenal hypoplasia congenita (AHC) and hypogonadotropic hypogonadism. Its expression is observed in adrenal tissues, gonads, and specific brain regions. NR0B1/DAX1 antibodies are essential tools for studying protein localization, expression patterns, and functional mechanisms in developmental biology and endocrine research. These antibodies are widely used in techniques like immunohistochemistry, Western blotting, and immunofluorescence to investigate adrenal disorders, sex determination pathways, and reproductive system development. Commercial antibodies typically target conserved regions (e.g., C-terminal ligand-binding domain) to ensure specificity across human and model organisms. Research applications include exploring DAX1's dual role in stem cell maintenance/differentiation and its potential oncogenic effects in adrenocortical/gonadal tumors.
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