| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CP25 ; HOX3; HOX3C; Hoxc6;;HOX C6 |
| WB Predicted band size | Calculated MW: 27 kDa ; Observed MW: 26 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human HOX C6 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
+ +
以下是3篇关于HoxC6抗体的参考文献示例(部分信息为模拟虚构，仅供参考)：
1. **文献名称**: "HoxC6 Expression in Vertebrate Limb Development"
**作者**: Smith J, et al.
**摘要**: 本研究利用特异性HoxC6抗体，通过免疫组化技术揭示HoxC6蛋白在小鼠胚胎肢体芽中的梯度表达模式，证明其在肢体近远端分化中的调控作用，抗体特异性经敲除模型验证。
2. **文献名称**: "HoxC6 Antibody Validation for Tumor Profiling"
**作者**: Lee H, et al.
**摘要**: 开发并验证了一种高特异性兔源HoxC6多克隆抗体，通过Western blot和免疫荧光确认其在乳腺癌组织中的选择性标记，发现HoxC6高表达与患者淋巴结转移呈正相关。
3. **文献名称**: "Role of HoxC6 in Neural Crest Cell Migration"
**作者**: García-Rojas M, et al.
**摘要**: 使用HoxC6抗体追踪鸡胚神经嵴细胞，发现该蛋白通过调控Slit-Robo通路影响细胞迁移路径，抗体特异性通过siRNA敲降实验的蛋白表达减弱得到证实。
注：实际文献需通过PubMed/Google Scholar检索关键词(如“HoxC6 antibody validation”或“HoxC6 immunohistochemistry”)获取。若研究较少，可扩展至相关Hox蛋白抗体的交叉引用文献。
The HoxC6 antibody is a specialized tool used to detect the HOXC6 protein, a member of the homeobox (HOX) gene family. HOX genes encode transcription factors critical for embryonic development, particularly in establishing body patterning and cell differentiation. The HOXC6 gene, located on chromosome 12 in humans, is part of the HOXC cluster and plays roles in skeletal, neural, and urogenital development. Dysregulation of HOXC6 has been implicated in cancers, including prostate, breast, and colorectal cancers, where it may act as an oncogene or biomarker.
The antibody targets specific epitopes of the HOXC6 protein, typically generated using recombinant HOXC6 protein fragments or synthetic peptides as immunogens. It is commonly produced in hosts like rabbits or mice and validated for applications such as Western blotting (WB), immunohistochemistry (IHC), immunofluorescence (IF), and chromatin immunoprecipitation (ChIP). Researchers utilize this antibody to study HOXC6's expression patterns, localization, and interactions in developmental and disease contexts. For instance, studies have linked HOXC6 overexpression to tumor progression, adipocyte regulation, and limb development.
Validation of antibody specificity often involves knockout controls or siRNA-mediated knockdown. Commercial HoxC6 antibodies vary in clonality (monoclonal/polyclonal), conjugation, and host species, requiring optimization for experimental conditions. Its utility spans basic developmental biology, cancer research, and regenerative medicine, aiding in mechanistic insights and potential therapeutic targeting. However, cross-reactivity with homologous HOX proteins remains a consideration, emphasizing the need for rigorous validation in each experimental system.
×
