| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Gtf2e1; TF2E1; TFIIE alpha;;TFIIE alpha |
| WB Predicted band size | Calculated MW: 49 kDa ; Observed MW: 60 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human TFIIE alpha |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于TFIIEα抗体的3篇参考文献及其简要摘要：
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1. **"Purification and characterization of transcription factor IIE from human cells"**
- **作者**: Ohkuma Y., Roeder R.G.
- **摘要**: 该研究从HeLa细胞中纯化了TFIIE蛋白复合物，并制备了针对TFIIEα亚基的特异性抗体。通过免疫印迹和功能实验，证实抗体能够有效抑制体外转录系统的活性，揭示了TFIIE在RNA聚合酶II转录起始中的必要作用。
2. **"Mutations in the human transcription elongation factor TFIIEα lead to impaired RNA polymerase II transcription"**
- **作者**: Yamaguchi Y., Wada T., Handa H.
- **摘要**: 文章通过定点突变分析TFIIEα的功能域，利用抗体验证突变蛋白的表达及稳定性。发现TFIIEα的酸性区域对转录延伸至关重要，抗体阻断实验进一步支持其与RNA聚合酶II磷酸化状态的调控相关。
3. **"Structural and functional analysis of TFIIE in the context of the RNA polymerase II transcription machinery"**
- **作者**: Tirode F., Busso D., Coin F., Egly J.M.
- **摘要**: 结合X射线晶体学和免疫共沉淀技术，该研究解析了TFIIEα与RNA聚合酶II的相互作用界面。抗体的使用帮助定位TFIIEα在转录复合体中的空间构象，阐明其介导转录起始向延伸转换的结构基础。
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这些文献涵盖了TFIIEα抗体的开发、功能验证及在转录机制研究中的应用，适用于分子生物学及基因表达调控领域的研究参考。
The TFIIEα antibody is a crucial tool in molecular biology for studying the role of TFIIEα, a subunit of the general transcription factor TFIIE, which is essential for RNA polymerase II-mediated transcription. TFIIE, composed of α (TFIIEα) and β (TFIIEβ) subunits, facilitates transcription initiation by stabilizing the pre-initiation complex, promoting TFIIH kinase activity, and regulating promoter escape. Specifically, TFIIEα interacts with RNA polymerase II and other transcription factors, bridging promoter recognition and the transition to elongation. Its dynamic involvement in DNA repair and transcription-coupled repair further highlights its functional versatility.
Antibodies targeting TFIIEα enable researchers to investigate its expression, localization, and interactions in diverse experimental contexts, including Western blotting, immunoprecipitation, chromatin immunoprecipitation (ChIP), and immunofluorescence. These applications help elucidate TFIIEα's role in transcriptional regulation, cell cycle progression, and stress responses. Dysregulation of TFIIEα has been implicated in diseases like cancer and developmental disorders, making its study clinically relevant. For instance, altered TFIIEα expression may disrupt transcription fidelity, contributing to oncogenesis. The antibody's specificity ensures accurate detection, aiding mechanistic studies of gene expression control and potential therapeutic targeting. Commercial TFIIEα antibodies are typically validated across species (e.g., human, mouse) and sample types (cells, tissues), supporting broad research utility in both basic and translational biomedical fields.
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