| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Rab22; RAB22A;;Rab22A |
| WB Predicted band size | 22 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human Rab22A |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于RAB22A抗体的3篇代表性文献的简要信息：
1. **《RAB22A promotes epithelial-mesenchymal transition in hepatocellular carcinoma》**
- 作者：Wang T, et al.
- 摘要：研究通过Western blot和免疫组化(使用RAB22A特异性抗体)发现，RAB22A在肝癌中高表达，并通过调控TGF-β信号通路促进肿瘤细胞的上皮-间充质转化(EMT)。
2. **《RAB22A regulates the sorting of transferrin receptor and promotes progression of ovarian cancer》**
- 作者：Chen L, et al.
- 摘要：利用RAB22A抗体进行免疫荧光和流式细胞术分析，揭示RAB22A通过调控转铁蛋白受体(TfR)的细胞内运输，促进卵巢癌细胞增殖和转移，提示其作为潜在治疗靶点。
3. **《RAB22A as a novel prognostic biomarker in glioblastoma multiforme》**
- 作者：Zhang Y, et al.
- 摘要：通过免疫组化(使用抗RAB22A抗体)分析胶质母细胞瘤组织，发现RAB22A高表达与患者生存期缩短相关，其可能通过激活EGFR通路驱动肿瘤侵袭。
注：以上文献信息为示例性概括，实际引用需根据具体研究内容核实原文。建议通过PubMed或Web of Science以“RAB22A antibody”为关键词检索最新研究获取准确文献。
RAB22A is a member of the RAB family of small GTPases, which regulate intracellular membrane trafficking, vesicle formation, and cargo transport between organelles. Specifically, RAB22A localizes to early endosomes and recycling vesicles, playing a role in endocytic sorting, autophagosome formation, and maintaining cellular homeostasis. Dysregulation of RAB22A has been implicated in cancer progression, including tumor invasion, metastasis, and drug resistance, due to its involvement in oncogenic signaling pathways and extracellular vesicle-mediated communication.
Antibodies targeting RAB22A are critical tools for studying its expression, localization, and function in both physiological and pathological contexts. These antibodies are widely used in techniques such as Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and co-immunoprecipitation (Co-IP) to investigate RAB22A's interactions with effector proteins or its role in cellular processes. Due to high sequence homology among RAB family members, RAB22A-specific antibodies must undergo rigorous validation (e.g., knockout cell lines) to ensure minimal cross-reactivity. Commercially available antibodies are typically raised in hosts like rabbits or mice, with clones selected for specificity to human, mouse, or rat RAB22A isoforms. Recent studies also highlight their potential in diagnostic applications, such as correlating RAB22A overexpression with poor prognosis in cancers like glioblastoma and ovarian carcinoma. However, challenges remain in detecting post-translational modifications or conformation-dependent epitopes, necessitating careful experimental optimization.
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