| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | nfe2 ; Nuclear factor; p45 NFE2;;NFE2 |
| WB Predicted band size | Calculated MW: 41 kDa ; Observed MW: 45 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human NFE2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于NFE2抗体的3篇模拟参考文献，供参考：
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1. **文献名称**：*NF-E2 is essential for megakaryocyte maturation and platelet formation*
**作者**：Shivdasani RA, et al.
**摘要**：本研究通过免疫荧光和Western blot技术，利用NFE2特异性抗体证实了NF-E2转录因子在巨核细胞分化中的关键作用。结果显示，NFE2缺失导致巨核细胞成熟障碍和血小板生成减少，提示其在造血系统中的核心调控功能。
2. **文献名称**：*DNA-binding specificity of the NFE2 transcription factor complex*
**作者**：Andrews NC, et al.
**摘要**：通过电泳迁移率变动实验(EMSA)和染色质免疫沉淀(ChIP)，结合NFE2抗体，本文揭示了NFE2与靶基因启动子区域的特异性结合模式，并阐明了其在小亚基(p18)和大亚基(p45)复合物形成中的分子机制。
3. **文献名称**：*A novel NFE2 mutation in congenital thrombocytopenia patients*
**作者**：Geddis AE, et al.
**摘要**：本研究在先天性血小板减少症患者中发现了NFE2基因的新发突变。通过免疫印迹和免疫组化分析(使用NFE2抗体)，证实突变导致蛋白稳定性下降，进而破坏巨核细胞分化，为临床诊断提供了分子标志物。
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*注：以上文献及作者为虚拟示例，实际研究中请通过PubMed或专业数据库检索具体文献。*
The nuclear factor erythroid 2 (NFE2) antibody is a tool used to study the NFE2 transcription factor, a member of the CNC-bZIP family that includes NRF1. NRF2. and NRF3. NFE2 plays a critical role in regulating erythroid and megakaryocytic differentiation, particularly in hematopoiesis. It binds to antioxidant response elements (AREs) in DNA, influencing the expression of genes involved in heme biosynthesis, globin production, and platelet formation. Structurally, NFE2 consists of a p45 subunit, which heterodimerizes with small Maf proteins (e.g., MafG) to activate or repress target genes.
Research on NFE2 has highlighted its involvement in disorders such as myeloproliferative neoplasms (MPNs), where mutations or dysregulation may contribute to abnormal blood cell proliferation. Antibodies targeting NFE2 are essential for detecting its expression and localization in cells or tissues via techniques like Western blotting, immunohistochemistry, or ChIP-seq. These studies help elucidate its role in oxidative stress responses, erythroid maturation, and disease mechanisms.
Interest in NFE2 antibodies also stems from their potential diagnostic and therapeutic applications, particularly in understanding conditions like thrombocytopenia or cancers with disrupted erythropoiesis. However, NFE2's functional overlap with other NRF family members, such as NRF2 (a key regulator of antioxidant defenses), necessitates careful experimental validation to ensure specificity in antibody-based assays. Ongoing studies aim to clarify NFE2's distinct pathways and interactions, advancing its relevance in hematology and molecular biology.
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