| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | RPS8;;RPS8 |
| WB Predicted band size | 24 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human RPS8 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于RPS8抗体的模拟参考文献示例(仅供参考，非真实文献)：
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1. **文献名称**：*Ribosomal Protein S8 as a Novel Biomarker in Colorectal Cancer: Expression Analysis Using a Specific Polyclonal Antibody*
**作者**：Smith A, et al.
**摘要**：本研究开发了一种针对RPS8的多克隆抗体，通过免疫组化和Western blot验证其在结直肠癌组织中的特异性。结果显示，RPS8在肿瘤组织中显著高表达，提示其可能作为癌症进展的生物标志物。
2. **文献名称**：*Autoantibodies Against RPS8 in Systemic Lupus Erythematosus: Clinical Correlations*
**作者**：Chen L, et al.
**摘要**：通过ELISA和免疫荧光技术检测系统性红斑狼疮(SLE)患者血清中的RPS8自身抗体。研究发现，RPS8抗体阳性与SLE患者的肾脏损伤和疾病活动度显著相关，为疾病分型提供了新依据。
3. **文献名称**：*Functional Characterization of RPS8 in Ribosome Biogenesis Using CRISPR/Cas9 and Antibody-Based Knockdown*
**作者**：Garcia R, et al.
**摘要**：利用CRISPR/Cas9和RPS8特异性抗体研究RPS8在核糖体合成中的功能。实验表明，RPS8缺失导致核糖体亚基组装异常，影响细胞增殖和蛋白质翻译效率。
4. **文献名称**：*RPS8 Antibody Cross-Reactivity in Phylogenetic Studies: Insights from Vertebrate Models*
**作者**：Wang Y, et al.
**摘要**：评估RPS8抗体在不同脊椎动物(小鼠、斑马鱼、人类)中的交叉反应性。研究发现抗体在哺乳动物中特异性较高，但在鱼类中表现交叉反应，提示其在进化研究中的应用需谨慎优化。
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注：以上为模拟生成的示例，实际文献需通过PubMed、Google Scholar等平台检索。如需真实文献，建议使用关键词“RPS8 antibody”“ribosomal protein S8”结合研究领域(如癌症、自身免疫)进一步筛选。
The Ribosomal Protein S8 (RPS8) antibody is a tool used to study the structure and function of the 40S ribosomal subunit, a critical component of the eukaryotic translation machinery. RPS8. encoded by the RPS8 gene in humans, is a core protein of the small ribosomal subunit, contributing to ribosome assembly and mRNA decoding. It plays a role in maintaining ribosomal stability and ensuring accurate protein synthesis. Antibodies targeting RPS8 are widely employed in molecular biology to investigate ribosomal biogenesis, cellular stress responses, and dysregulation in diseases linked to ribosomopathies, such as Diamond-Blackfan anemia or certain cancers.
These antibodies are commonly used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to detect RPS8 expression levels or localization. Researchers also utilize them to explore connections between ribosomal dysfunction and pathological conditions, including tumorigenesis, where altered ribosome production may drive uncontrolled cell proliferation. Additionally, RPS8 antibodies occasionally serve as loading controls in protein analysis due to the protein’s relatively stable expression under standard conditions.
Commercial RPS8 antibodies are typically raised in hosts like rabbits or mice, with validation via knockout cell lines or siRNA-mediated silencing. Their specificity makes them valuable for studying translational regulation, cellular growth, and ribosome-related genetic disorders.
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