| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | MIMT44; TIM44;;TIM44 |
| WB Predicted band size | Calculated MW: 51 kDa ; Observed MW: 45 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Rat |
| Immunogen | A synthesized peptide derived from human TIM44 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是3篇与TIM44抗体相关的文献示例(内容基于真实研究概括，但文献标题和作者为示例性描述)：
1. **文献名称**：The mitochondrial import machinery: TIM44 as a central component in protein translocation
**作者**：Neupert W, et al.
**摘要**：研究揭示了TIM44在线粒体蛋白质导入过程中的核心作用，通过抗体标记实验证实其与TIM复合物结合，协助前体蛋白跨线粒体内膜的转位。
2. **文献名称**：Functional interaction of TIM44 with mitochondrial HSP70 during protein import
**作者**：Horst M, et al.
**摘要**：利用TIM44特异性抗体进行共免疫沉淀实验，证明TIM44与线粒体HSP70协同作用，为前体蛋白的折叠提供能量驱动机制。
3. **文献名称**：Role of TIM44 in human mitochondrial diseases: Antibody-based diagnostic approaches
**作者**：Zhao Q, et al.
**摘要**：通过开发高特异性TIM44抗体，研究发现某些线粒体疾病患者中TIM44表达异常，提示其作为潜在生物标志物的价值。
注：以上文献名为示例，实际研究需通过PubMed等数据库检索(如PMID: 12345678)。建议使用关键词“TIM44 antibody mitochondrial import”或结合具体研究领域筛选。
TIM44 (Translocase of the Inner Mitochondrial Membrane 44) is a key component of the mitochondrial protein import machinery, primarily involved in the transport of nuclear-encoded proteins into the mitochondrial matrix. It acts as a peripheral membrane protein anchored to the inner mitochondrial membrane, where it serves as a critical adaptor between the translocase TIM23 complex and the mitochondrial Hsp70 (mtHsp70) chaperone system. TIM44 facilitates the ATP-dependent translocation of precursor proteins by recruiting mtHsp70 to the translocation channel, enabling the binding and folding of incoming polypeptides.
Structurally, TIM44 contains an N-terminal transmembrane domain and a C-terminal acidic domain that interacts with mtHsp70. Its dynamic association with TIM23 and coordination with co-chaperones like Tim14 (DNAJA3) ensure efficient protein import, a process vital for mitochondrial biogenesis and function. Dysregulation of TIM44 has been linked to mitochondrial disorders and cellular stress responses.
TIM44 antibodies are essential tools for studying mitochondrial protein import mechanisms, protein-protein interactions, and mitochondrial homeostasis. They are widely used in techniques such as Western blotting, immunofluorescence, and co-immunoprecipitation to detect TIM44 expression, localization, and its interactions within the TIM23 complex. These antibodies also aid in exploring pathological conditions associated with mitochondrial dysfunction, including neurodegenerative diseases and cancer.
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