| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SSR2 |
| Uniprot No | P43308 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 18-149aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSEEGARLL ASKSLLNRYA VEGRDLTLQY NIYNVGSSAA LDVELSDDSF PPEDFGIVSG MLNVKWDRIA PASNVSHTVV LRPLKAGYFN FTSATITYLA QEDGPVVIGS TSAPGQGGIL AQREFDRRFS PHFLD |
| 预测分子量 | 17 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是假设性的参考文献示例，仅供参考，实际文献需通过学术数据库验证：
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1. **文献名称**：*Expression and functional analysis of recombinant SSR2 in protein translocation*
**作者**：Müller, G., et al.
**摘要**：研究利用大肠杆菌系统表达SSR2重组蛋白，证实其参与内质网信号肽识别及转运复合体组装，缺失实验显示转运功能受损。
2. **文献名称**：*Structural characterization of human SSR2 using mammalian cell expression systems*
**作者**：Sekizuka, T., & Yamaguchi, A.
**摘要**：通过哺乳动物细胞表达纯化SSR2.结合冷冻电镜解析其与转运通道蛋白的相互作用，揭示跨膜结构域对底物识别的关键作用。
3. **文献名称**：*SSR2 knockdown and recombinant rescue in cancer cell models*
**作者**：Nakamura, Y., et al.
**摘要**：发现SSR2在胃癌细胞中高表达，通过重组SSR2蛋白回补实验逆转了基因敲低导致的分泌蛋白运输阻滞，提示其潜在治疗靶点价值。
4. **文献名称**：*Crystallization and X-ray analysis of recombinant SSR2 core domain*
**作者**：Johnson, R.L., & Smith, C.A.
**摘要**：报道SSR2重组蛋白核心结构域的结晶条件及初步X射线衍射数据，为后续机制研究提供结构基础。
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**提示**：以上为模拟示例，建议通过PubMed、Web of Science等平台以“SSR2 recombinant protein”或“Signal Sequence Receptor 2”为关键词检索真实文献。
**Background of SSR2 Recombinant Protein**
The SSR2 (Signal Sequence Receptor 2) recombinant protein is a key component of the translocon-associated protein (TRAP) complex, which is integral to protein translocation across the endoplasmic reticulum (ER) membrane in eukaryotic cells. SSR2. also known as TRAPβ, functions as a transmembrane subunit of the TRAP complex, assisting in the recognition and transport of nascent polypeptides during their co-translational insertion into the ER. This process is critical for the proper folding, modification, and secretion of secretory and membrane proteins.
The recombinant SSR2 protein is engineered using molecular cloning techniques, where the *SSR2* gene is expressed in heterologous systems like *E. coli* or mammalian cell cultures. This allows for large-scale production of the protein with high purity, enabling structural and functional studies. Recombinant SSR2 retains its ability to interact with other translocon components, such as the Sec61 channel, and plays a role in stabilizing the ribosome-translocon junction during protein translocation.
Research on SSR2 has shed light on its involvement in diseases, including certain cancers and neurodegenerative disorders, where ER stress or protein misfolding occurs. Its recombinant form is widely used in *in vitro* assays to study protein translocation mechanisms, screen for ER-targeting therapeutics, or analyze mutations affecting TRAP complex functionality. Structural studies using recombinant SSR2. combined with cryo-EM or X-ray crystallography, have further elucidated its role in the dynamic translocon machinery.
Overall, SSR2 recombinant protein serves as a vital tool for dissecting ER-related biological processes and developing strategies to address pathologies linked to protein transport dysregulation.
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