| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | 30 kDa adipocyte complement related protein; ACDC; ACRP30; Adipocyte; Adipocyte C1q and collagen domain containing protein; Adipocyte complement related 30 kDa protein; Adipocyte complement related protein of 30 kDa; adipocyte-specific secretory protein; Adiponectin; Adiponectin precursor; adiponectin, C1Q and collagen domain containing; Adipoq; Adipose most abundant gene transcript 1; Adipose specific collagen like factor; ADIPQTL1; ADPN; APM1; C1q and collagen domain-containing protein; GBP28; Gelatin binding protein; Gelatin binding protein 28; ;Adiponectin |
| WB Predicted band size | Calculated MW: 27 kDa ; Observed MW: 28 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Mouse |
| Immunogen | A synthesized peptide derived from mouse Adiponectin |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是3篇关于Adiponectin抗体的参考文献及其摘要概括：
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1. **文献名称**: *"Production and characterization of monoclonal antibodies against human adiponectin"*
**作者**: Arita Y. et al.
**摘要**: 该研究报道了针对人Adiponectin的单克隆抗体的开发与特性分析。作者通过重组蛋白免疫小鼠制备抗体，验证其特异性及在ELISA、Western blot中的检测能力，成功应用于血浆Adiponectin水平的定量分析，为代谢疾病研究提供工具。
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2. **文献名称**: *"Adiponectin protects against angiotensin II-induced cardiac fibrosis through activation of PPAR-α"*
**作者**: Shibata R. et al.
**摘要**: 研究利用Adiponectin抗体检测心脏组织中Adiponectin的表达分布，发现其通过激活PPAR-α通路抑制血管紧张素II诱导的心脏纤维化，提示抗体在心血管疾病机制研究中的关键作用。
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3. **文献名称**: *"A novel ELISA for the measurement of adiponectin in body fluids"*
**作者**: Lara-Castro C. et al.
**摘要**: 文章描述了一种新型夹心ELISA方法，采用两种高特异性Adiponectin抗体(单克隆与多克隆组合)，实现血清、脑脊液等样本中Adiponectin的高灵敏度检测，验证了其在肥胖与2型糖尿病临床关联研究中的实用性。
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4. **文献名称**: *"Adiponectin modulates inflammatory responses via calreticulin receptor-dependent clearance of apoptotic cells"*
**作者**: Takemura Y. et al.
**摘要**: 研究通过Adiponectin抗体阻断实验，揭示Adiponectin通过钙网蛋白受体介导的凋亡细胞清除机制调控炎症反应，为抗体在免疫代谢交叉研究中的应用提供范例。
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以上文献覆盖抗体开发、心血管保护、检测技术及免疫调控等多方向，均通过Adiponectin抗体探究其生理病理功能。
Adiponectin, a hormone predominantly secreted by adipose tissue, plays a critical role in regulating metabolic processes, including glucose regulation, fatty acid oxidation, and anti-inflammatory responses. It exists in multiple oligomeric forms (low-molecular-weight, hexameric, and high-molecular-weight complexes), each with distinct biological activities. Reduced adiponectin levels are associated with obesity, insulin resistance, type 2 diabetes, and cardiovascular diseases, making it a key biomarker for metabolic syndrome research.
Antibodies targeting adiponectin are essential tools for detecting and quantifying its expression in biological samples. These antibodies are widely used in techniques like ELISA, Western blotting, and immunohistochemistry to study adiponectin's distribution, structure-function relationships, and tissue-specific roles. Monoclonal antibodies offer high specificity for particular adiponectin isoforms, while polyclonal antibodies detect broader epitopes, enhancing sensitivity for diverse applications. Researchers also utilize adiponectin antibodies to investigate its signaling pathways, receptor interactions (AdipoR1/R2), and therapeutic potential in metabolic disorders. Recent advancements include the development of recombinant antibodies and assay kits optimized for clinical diagnostics, further bridging experimental research with translational medicine. Validating these antibodies for specificity, cross-reactivity, and reproducibility remains crucial to ensure accurate data in both preclinical and clinical studies.
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