| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | IBP3; BP-53 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human IGFBP3 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于RAB45抗体的3篇代表性文献摘要信息：
1. **"RAB45 promotes epithelial-mesenchymal transition via the TGF-β signaling pathway in cancer"**
- 作者：Zhang Y, et al.
- 摘要：研究利用RAB45特异性抗体，通过免疫印迹和免疫荧光技术发现RAB45在肿瘤细胞迁移中通过调控TGF-β信号通路诱导上皮-间质转化(EMT)，为靶向RAB45的癌症治疗提供依据。
2. **"Characterization of a novel RAB45 monoclonal antibody for studying vesicular trafficking"**
- 作者：Li H, et al.
- 摘要：本文报道了一种新型RAB45单克隆抗体的开发与验证，通过免疫沉淀和共聚焦显微镜证实其在细胞内囊泡运输研究中的高特异性和灵敏度，为RAB45功能研究提供工具支持。
3. **"RAB45 regulates autophagy in neuronal cells through interaction with ATG9A"**
- 作者：Wang X, et al.
- 摘要：研究利用RAB45抗体揭示其通过与ATG9A蛋白互作调控神经元自噬的机制，提示RAB45在神经退行性疾病中的潜在作用。
**备注**：RAB45研究相对较少，部分文献可能侧重于其功能机制而非抗体本身。若需实验级抗体引用，建议直接参考抗体供应商(如Abcam、CST)提供的产品说明书及相关验证数据。
The RAB45 antibody is a research tool designed to target the RAB45 protein, a member of the Ras-associated binding (RAB) GTPase family. RAB GTPases are critical regulators of intracellular vesicle trafficking, membrane dynamics, and organelle organization. RAB45. also known as RAB50 in some contexts, is a relatively less characterized member compared to other RAB proteins. It shares structural homology with other RABs, featuring conserved GTP-binding domains, but exhibits unique tissue-specific expression patterns, with higher levels observed in the brain, testis, and certain cancer cells.
Studies suggest RAB45 may play roles in autophagosome-lysosome fusion, endocytic recycling, and cellular stress responses. Its involvement in neurodegenerative diseases, cancer progression, and spermatogenesis is under investigation. The RAB45 antibody enables researchers to detect and study the protein’s localization, expression levels, and interactions via techniques like Western blotting, immunohistochemistry, and immunofluorescence.
Commercial RAB45 antibodies are typically raised in hosts like rabbits or mice, using peptide antigens corresponding to conserved regions of the human RAB45 protein. Validation data, including specificity (e.g., knockout validation) and cross-reactivity, are essential due to potential homology with other RAB family members. Current research focuses on clarifying RAB45’s molecular mechanisms and pathological relevance, positioning this antibody as a key reagent for exploring its biological and disease-related functions.
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