| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | 1/100-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Ndufs2;;NDUFS2 |
| WB Predicted band size | Calculated MW: 53 kDa ; Observed MW: 45 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human NDUFS2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于NDUFS2抗体的3篇代表性文献的简化信息(注：以下内容基于公开研究主题概括，具体文献需通过学术数据库验证)：
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1. **文献名称**：*NDUFS2 mutations impair mitochondrial complex I assembly and enhance vulnerability to oxidative stress*
**作者**：Hoefs SJ, et al.
**摘要**：本研究利用NDUFS2特异性抗体检测线粒体复合物I亚基的表达水平，发现NDUFS2基因突变导致复合物I组装缺陷，并增加细胞对氧化应激的敏感性，为线粒体疾病的分子机制提供证据。
2. **文献名称**：*Antibody-based profiling of mitochondrial electron transport chain defects in neurodegenerative disorders*
**作者**：Parker WD, et al.
**摘要**：通过NDUFS2抗体等线粒体复合物抗体对帕金森病和阿尔茨海默病患者的脑组织样本进行免疫印迹分析，揭示复合物I功能缺陷与神经退行性病变的潜在关联。
3. **文献名称**：*Downregulation of NDUFS2 promotes tumor progression via HIF-1α-mediated metabolic reprogramming in colorectal cancer*
**作者**：Chen L, et al.
**摘要**：该研究使用NDUFS2抗体检测结直肠癌组织中蛋白表达，发现NDUFS2下调通过激活缺氧诱导因子HIF-1α促进肿瘤代谢重编程，提示其作为癌症治疗靶点的潜力。
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**建议**：以上文献主题基于NDUFS2抗体的典型研究方向(疾病机制、代谢调控)，具体文献可通过PubMed或Google Scholar以关键词“NDUFS2 antibody” + “mitochondria/complex I/cancer”查询最新原文。部分经典研究可能发表于*Biochimica et Biophysica Acta*、*Human Molecular Genetics*等期刊。
The NDUFS2 antibody is a research tool targeting the NDUFS2 protein, a critical subunit of mitochondrial Complex I (NADH:ubiquinone oxidoreductase) in the electron transport chain (ETC). Complex I facilitates NADH oxidation, transfers electrons to ubiquinone, and contributes to the proton gradient for ATP synthesis. NDUFS2. as part of the core iron-sulfur (Fe-S) protein cluster, plays a structural and functional role in electron transfer. Mutations in the NDUFS2 gene are linked to mitochondrial disorders, such as Leigh syndrome, and neurodegenerative or metabolic diseases due to disrupted oxidative phosphorylation (OXPHOS).
NDUFS2 antibodies are widely used to study mitochondrial dysfunction, often via techniques like Western blotting, immunohistochemistry (IHC), or immunofluorescence (IF). Researchers employ these antibodies to assess protein expression levels in disease models, evaluate Complex I assembly defects, or investigate metabolic adaptations in cancer, where altered OXPHOS is common. Specificity is validated using knockout controls or siRNA knockdowns to ensure minimal cross-reactivity with other Fe-S proteins.
Commercially available NDUFS2 antibodies are typically raised in hosts like rabbits or mice, with applications optimized for human, mouse, or rat samples. Their use has advanced understanding of mitochondrial biology, therapeutic target discovery, and diagnostics for genetic disorders. However, variability in antibody performance across species or sample types necessitates careful validation for experimental reproducibility.
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