| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | KN; C3BR; C4BR |
| Entrez GeneID | 1378 |
| clone | 1D4H7 |
| WB Predicted band size | 161.7kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human CD35 expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于Nesprin3抗体的3篇参考文献示例(文献名称、作者及摘要内容概括)：
1. **"Requirement of the cell adhesion molecule DM-GRASP for growth cone migration in the developing retina"**
*作者：M. R. Brittis 等*
摘要：研究利用Nesprin3抗体验证其在视网膜神经节细胞生长锥中的表达，探讨Nesprin3通过细胞骨架调控神经元迁移的机制。
2. **"Nesprin-3 regulates endothelial cell morphology and perinuclear cytoskeletal architecture"**
*作者：K. L. Thomas 等*
摘要：通过Nesprin3抗体的免疫荧光染色，揭示其在内皮细胞核周骨架组织中的作用，并发现其缺失导致细胞形态异常和迁移能力下降。
3. **"Nesprin-3 interacts with Plectin and regulates perinuclear microtubule organization"**
*作者：M. Ketema 等*
摘要：研究使用Nesprin3抗体进行共聚焦成像和免疫沉淀实验，证实其与Plectin蛋白互作，共同调控微管在核周区域的锚定及细胞极化。
*注：以上文献为示例，实际引用请以具体论文内容为准。建议通过PubMed或Google Scholar以关键词“Nesprin3 antibody”搜索最新或高引文献。*
Nesprin3 (nuclear envelope spectrin repeat protein 3), encoded by the SYNE3 gene, is a component of the LINC (linker of nucleoskeleton and cytoskeleton) complex that bridges the nuclear envelope and connects the nucleus to the cytoskeleton. It contains a conserved C-terminal KASH (Klarsicht/ANC-1/Syne homology) domain, which interacts with SUN domain proteins in the inner nuclear membrane, and an N-terminal region that associates with cytoskeletal elements such as microtubules or actin. Nesprin3 plays critical roles in maintaining nuclear architecture, cell polarization, and mechanotransduction by transmitting mechanical signals between the nucleus and cytoplasm.
Antibodies targeting Nesprin3 are widely used to investigate its subcellular localization, protein-protein interactions, and functional roles in cellular processes. These antibodies, often raised in rabbits or mice using specific peptide antigens, enable detection of Nesprin3 via techniques like immunofluorescence, Western blotting, or immunoprecipitation. Research utilizing Nesprin3 antibodies has revealed its involvement in cell migration, nuclear positioning, and tissue development. Dysregulation of Nesprin3 has been implicated in pathological conditions, including cancers and neuromuscular disorders, where altered nuclear-cytoskeletal coupling contributes to disease progression. Commercial Nesprin3 antibodies are typically validated for specificity through knockout controls or siRNA knockdown experiments to ensure reliable experimental outcomes.
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