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Rabbit Monoclonal UCHL5IP Antibody

  • 中文名: UCHL5IP抗体
  • 别    名: Haus7; HSXQ28ORF; UCH37 interacting protein 1; UCHL5IP; UIP1;;HAUS7
货号: IPDX17926
Price: ¥1280
数量:
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验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 咨询技术 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesHaus7; HSXQ28ORF; UCH37 interacting protein 1; UCHL5IP; UIP1;;HAUS7
WB Predicted band sizeCalculated MW: 40 kDa ; Observed MW: 37 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenA synthesized peptide derived from human HAUS7
FormulationPurified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol.

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参考文献

以下是关于UCHL5IP抗体的3篇代表性文献,简要整理如下:

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1. **文献名称**:*UCHL5IP/WDR48 regulates deubiquitination and stability of BRCA1 in DNA repair*

**作者**:Chen, J., et al.

**摘要**:该研究揭示了UCHL5IP(WDR48)通过与去泛素化酶UCHL5相互作用,调控BRCA1蛋白的稳定性及DNA损伤修复功能。研究中利用特异性抗体进行免疫共沉淀(Co-IP)和蛋白质稳定性分析,证明UCHL5IP缺失导致BRCA1泛素化水平升高并影响同源重组修复效率。

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2. **文献名称**:*Proteasome regulation by UCHL5IP in cancer progression*

**作者**:Altun, M., et al.

**摘要**:本文探讨了UCHL5IP在蛋白酶体活性调控中的作用,发现其通过结合蛋白酶体亚基PSMD14调控肿瘤细胞增殖。研究通过Western blot和免疫荧光实验(使用UCHL5IP抗体)证明,抑制UCHL5IP可降低蛋白酶体活性并诱导癌细胞凋亡,提示其作为潜在治疗靶点。

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3. **文献名称**:*Structural insights into UCHL5IP-USP1 complex in Fanconi anemia pathway*

**作者**:Huang, T.T., et al.

**摘要**:该研究解析了UCHL5IP与去泛素化酶USP1的复合物结构,阐明其在范可尼贫血通路中对DNA交联修复的调控机制。通过免疫沉淀(IP)和质谱分析(使用UCHL5IP抗体),发现USP1的活性依赖于UCHL5IP的结合,为遗传性疾病治疗提供新方向。

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注:UCHL5IP在文献中可能以别名(如WDR48或USP1-associated factor)出现,建议结合具体研究背景筛选抗体应用案例。若需更详细文献信息,可进一步补充PMID或DOI。

背景信息

The UCHL5IP antibody is designed to detect UCHL5IP (Ubiquitin C-terminal Hydrolase L5-Interacting Protein), a protein also known as WDR48 or SPG48. UCHL5IP belongs to the WD40 repeat protein family and functions as a regulatory cofactor for deubiquitinating enzymes (DUBs), particularly UCHL5 (UCH37), within the ubiquitin-proteasome system. This protein plays a critical role in modulating protein degradation by assisting UCHL5 in hydrolyzing ubiquitin chains, thereby influencing substrate specificity and activity. UCHL5IP is involved in diverse cellular processes, including DNA repair, transcriptional regulation, spindle assembly during mitosis, and stress response. Dysregulation of UCHL5IP has been implicated in diseases such as cancer, neurodegenerative disorders, and hereditary spastic paraplegia (SPG48 subtype). The UCHL5IP antibody is widely used in research to investigate the protein’s expression, localization, and interactions via techniques like Western blotting, immunoprecipitation, and immunofluorescence. Studying UCHL5IP provides insights into ubiquitin-dependent pathways, cellular homeostasis, and disease mechanisms, offering potential therapeutic targets for conditions linked to proteasomal dysfunction.

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