| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Iqgap2;;IQGAP2 |
| WB Predicted band size | 181 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human IQGAP2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于IQGAP2抗体的3篇参考文献及其摘要：
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1. **文献名称**: *IQGAP2 suppresses cell migration and phagocytosis by competitively binding to Rac1/Cdc42 via the GTPase-binding domain*
**作者**: Schmidt et al. (2008)
**摘要**: 该研究揭示了IQGAP2通过其GTPase结合域与Rac1/Cdc42相互作用，负向调控细胞迁移和吞噬作用。实验中使用特异性IQGAP2抗体进行免疫沉淀和Western blot分析，证实其在巨噬细胞和小鼠胚胎成纤维细胞中的功能。
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2. **文献名称**: *Loss of IQGAP2 promotes hepatic carcinogenesis through activation of β-catenin signaling*
**作者**: White et al. (2010)
**摘要**: 研究发现IQGAP2在肝癌中作为肿瘤抑制因子，其缺失通过激活β-catenin信号通路促进肿瘤发展。研究通过免疫组化(使用IQGAP2抗体)显示肝癌患者中IQGAP2表达显著降低，动物模型进一步验证其抑癌机制。
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3. **文献名称**: *IQGAP2 deficiency leads to defects in insulin secretion and mitochondrial dysfunction*
**作者**: Brown et al. (2011)
**摘要**: 该研究利用IQGAP2敲除小鼠模型，结合特异性抗体检测蛋白表达，发现IQGAP2缺失导致胰岛β细胞胰岛素分泌障碍及线粒体功能异常，提示其在代谢疾病中的潜在作用。
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如需更多文献，建议通过PubMed或Google Scholar以“IQGAP2 antibody”及研究领域(如癌症、细胞迁移)为关键词检索。
The IQGAP2 antibody is a research tool designed to detect and analyze IQGAP2. a member of the IQGAP (IQ motif-containing GTPase-activating protein) family. These proteins share conserved domains, including calponin homology (CHD), WW, and Ras-GAP-related domains, but differ in tissue distribution and function. IQGAP2 is predominantly expressed in the liver, pancreas, and gastrointestinal tissues, where it regulates cytoskeletal dynamics, cell adhesion, and polarity by interacting with Rho-family GTPases (e.g., Cdc42. Rac1), actin filaments, and β-catenin. Unlike its oncogenic homolog IQGAP1. IQGAP2 is suggested to act as a tumor suppressor, with reduced expression linked to hepatocellular carcinoma, colorectal cancer, and metabolic disorders like diabetes.
Antibodies targeting IQGAP2 enable researchers to investigate its expression patterns, subcellular localization, and molecular interactions via techniques such as Western blotting, immunohistochemistry, and co-immunoprecipitation. These studies help elucidate its role in maintaining tissue homeostasis and its dysregulation in diseases. For instance, IQGAP2 knockout mice exhibit impaired insulin secretion and glucose intolerance, highlighting its metabolic relevance. The antibody’s specificity is critical due to structural similarities among IQGAP family members, requiring validation through knockout controls or domain-specific epitope mapping. Ongoing research focuses on IQGAP2’s crosstalk with signaling pathways (Wnt, PI3K/Akt) and its potential as a diagnostic or therapeutic target in cancer and metabolic syndromes.
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