| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | B56D; PPP2R5D; Tex271;;PPP2R5D |
| WB Predicted band size | Calculated MW: 70 kDa ; Observed MW: 60,65 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human PPP2R5D |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于PPP2R5D抗体的3篇代表性文献示例(注：文献为虚拟示例，实际引用需检索数据库获取真实信息)：
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1. **文献名称**：*PPP2R5D regulates DNA damage response through dephosphorylation of ATM*
**作者**：Li X, et al.
**摘要**：本研究利用PPP2R5D特异性抗体，揭示了PPP2R5D作为PP2A复合体亚基通过去磷酸化ATM调控DNA损伤修复的机制，为癌症治疗提供潜在靶点。
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2. **文献名称**：*Development and validation of a monoclonal antibody for PPP2R5D in neurodevelopmental disorders*
**作者**：Smith J, et al.
**摘要**：报道了一种高特异性PPP2R5D单克隆抗体的开发，通过免疫印迹和免疫组化验证其在神经组织中的表达异常，支持其在自闭症相关突变研究中的应用。
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3. **文献名称**：*PPP2R5D-antibody complex structure reveals its role in PP2A substrate recognition*
**作者**：Chen H, et al.
**摘要**：通过冷冻电镜解析PPP2R5D抗体结合后的复合物结构，阐明其参与PP2A底物识别的分子机制，为设计靶向PP2A的抑制剂提供结构基础。
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如需真实文献，建议在PubMed或Google Scholar中搜索关键词 **"PPP2R5D antibody"** 或 **"PPP2R5D PP2A function"**，并筛选近年发表的研究。
**Background of PPP2R5D Antibody**
The PPP2R5D antibody targets the regulatory subunit B56δ (encoded by the *PPP2R5D* gene) of protein phosphatase 2A (PP2A), a serine/threonine phosphatase critical for cellular processes like cell cycle regulation, signal transduction, and apoptosis. PP2A holoenzymes consist of a catalytic subunit (C), a scaffolding subunit (A), and a variable regulatory B subunit. The B56 family (B’/PR61), including PPP2R5D, determines substrate specificity and subcellular localization.
PPP2R5D is implicated in dephosphorylating key signaling molecules, such as AKT and β-catenin, modulating pathways like Wnt and PI3K/AKT/mTOR. Dysregulation of PPP2R5D is linked to cancers, neurodegenerative disorders, and developmental defects. For instance, *PPP2R5D* mutations are associated with intellectual disability and autism spectrum disorders.
Antibodies against PPP2R5D are essential tools for studying its expression, interactions, and role in disease. They enable techniques like Western blotting, immunoprecipitation, and immunofluorescence to assess protein levels, localization, and post-translational modifications. Commercial PPP2R5D antibodies are typically validated for specificity using knockout cell lines or siRNA knockdown. Researchers use these antibodies to explore PPP2R5D's tumor-suppressive or oncogenic roles, depending on context, and its potential as a therapeutic target. Reliable detection of PPP2R5D aids in understanding PP2A complex dynamics and their impact on cellular homeostasis.
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