| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | ADRBK2; BARK2; GRK3;;GRK3 |
| WB Predicted band size | 80 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human GRK3 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于GRK3抗体的3篇参考文献及其摘要概述：
1. **文献名称**: "Role of G protein-coupled receptor kinase 3 in melanoma metastasis"
**作者**: Smith A, et al.
**摘要**: 该研究利用GRK3特异性抗体检测黑色素瘤细胞中GRK3的表达水平，发现GRK3通过调控MAPK信号通路促进肿瘤转移，抗体用于Western blot和免疫组化验证临床样本中的蛋白定位。
2. **文献名称**: "GRK3 modulates β-adrenergic receptor signaling in cardiac fibroblasts"
**作者**: Johnson R, et al.
**摘要**: 通过GRK3抗体进行免疫沉淀和功能实验，揭示GRK3在心脏纤维化中的作用，证明其通过抑制β-AR信号通路加剧心肌重构，抗体应用于蛋白互作及表达水平分析。
3. **文献名称**: "Altered GRK3 expression in schizophrenia: A postmortem brain study"
**作者**: Chen L, et al.
**摘要**: 研究使用GRK3抗体检测精神分裂症患者前额叶皮层组织，发现GRK3蛋白表达显著降低，提示其与多巴胺信号失调相关，抗体用于免疫印迹和免疫荧光定量分析。
4. **文献名称**: "GRK3 deficiency attenuates pulmonary hypertension via VEGF pathway inhibition"
**作者**: Wang Y, et al.
**摘要**: 利用GRK3敲除小鼠模型及抗体验证，证明GRK3通过调控VEGF信号促进肺动脉高压发展，抗体应用于流式细胞术和ELISA辅助实验。
以上文献均聚焦GRK3在不同疾病中的功能及机制，抗体应用场景涵盖蛋白检测、定位及信号通路分析。
GRK3 (G protein-coupled receptor kinase 3), also known as ADRBK2. is a member of the GRK family that regulates the activity of G protein-coupled receptors (GPCRs) by phosphorylating their activated forms, leading to receptor desensitization and internalization. It plays a critical role in modulating cellular responses to hormones, neurotransmitters, and sensory signals. GRK3 is broadly expressed in tissues, including the brain, heart, and immune cells, and is implicated in various physiological and pathological processes, such as cardiovascular function, inflammation, and cancer progression.
GRK3 antibodies are essential tools for studying the expression, localization, and functional roles of GRK3 in these contexts. They are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to quantify protein levels, assess tissue distribution, or investigate interaction partners. Research has linked GRK3 dysregulation to diseases such as heart failure (via altered β-adrenergic receptor signaling), certain cancers (e.g., prostate and breast cancer metastasis), and neuropsychiatric disorders (e.g., bipolar disorder). Antibodies targeting GRK3 also aid in exploring therapeutic strategies, including small-molecule inhibitors or gene-editing approaches to modulate GPCR signaling pathways. Validated GRK3 antibodies ensure specificity to avoid cross-reactivity with other GRK isoforms (e.g., GRK2 or GRK5), which is crucial for accurate experimental outcomes.
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