首页 / 产品 / ELISA试剂盒 / 兔ELISA试剂盒
产品描述：兔子白介素6(IL-6)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL6含量。IL6即白细胞介素6，是一种多功能细胞因子，在免疫调节、造血、炎症反应等生理和病理过程中发挥关键作用。其异常表达与多种疾病相关，如自身免疫病、癌症。研究机制主要聚焦于其信号通路，旨在开发阻断IL6活性的疗法，以治疗相关疾病。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于科研场景中炎症机制研究、疾病动物模型评估或药物干预效果分析本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：IL6Interleukin-6 ELISA Kit; IL-6 ELISA Kit
缩写：IL6
Uniprot No.：Q9MZR1
种属：Oryctolagus cuniculus (Rabbit)
样本类型：serum, plasma, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit IL-6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 88 | ||||
| Range % | 80-92 | |||||
| 1:2 | Average % | 99 | ||||
| Range % | 91-105 | |||||
| 1:4 | Average % | 101 | ||||
| Range % | 92-110 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of rabbit IL-6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 89-98 | ||||
| EDTA plasma (n=4) | 96 | 90-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 10002.8402.7852.8132.710 5002.3162.2232.2702.167 2501.5801.5671.5741.471 1250.9350.8740.9050.802 62.50.4790.4620.4710.368 31.20.2660.2540.2600.157 15.60.1700.1580.1640.061 00.1070.0990.103 | ||||||
货期：3-5 working days
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