| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SQSTM1 |
| Uniprot No | Q13501 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-440aa |
| 氨基酸序列 | MASLTVKAYLLGKEDAAREIRRFSFCCSPEPEAEAEAAAGPGPCERLLSR VAALFPALRPGGFQAHYRDEDGDLVAFSSDEELTMAMSYVKDDIFRIYIK EKKECRRDHRPPCAQEAPRNMVHPNVICDGCNGPVVGTRYKCSVCPDYDL CSVCEGKGLHRGHTKLAFPSPFGHLSEGFSHSRWLRKVKHGHFGWPGWEM GPPGNWSPRPPRAGEARPGPTAESASGPSEDPSVNFLKNVGESVAAALSP LGIEVDIDVEHGGKRSRLTPVSPESSSTEEKSSSQPSSCCSDPSKPGGNV EGATQSLAEQMRKIALESEGRPEEQMESDNCSGGDDDWTHLSSKEVDPST GELQSLQMPESEGPSSLDPSQEGPTGLKEAALYPHLPPEADPRLIESLSQ MLSMGFSDEGGWLTRLLQTKNYDIGAALDTIQYSKHPPPL |
| 预测分子量 | 74 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SQSTM1重组蛋白的3篇参考文献及简要摘要：
1. **"SQSTM1/p62: A key protein linking proteostasis to cancer"**
*作者：Moscat J, Diaz-Meco MT*
摘要：该综述总结了SQSTM1/p62在细胞自噬和泛素-蛋白酶体系统中的作用，强调其通过结合泛素化蛋白和LC3促进异常蛋白聚集体的降解，并探讨其在肿瘤发生中的双重调控机制。
2. **"Structural basis for sorting mechanism of p62 in selective autophagy"**
*作者：Ichimura Y, Kumanomidou T, et al.*
摘要：研究通过X射线晶体学解析SQSTM1的PB1和UBA结构域，揭示其通过寡聚化形成多价支架，特异性识别泛素化底物并介导自噬体形成的分子机制。
3. **"Loss of autophagy in the central nervous system causes neurodegeneration in mice"**
*作者：Komatsu M, Waguri S, et al.*
摘要：利用SQSTM1敲除小鼠模型，发现其脑部出现泛素化蛋白异常沉积及神经元退行性病变，证实SQSTM1通过自噬途径维持神经细胞内蛋白质稳态的关键作用。
4. **"p62/SQSTM1 binds directly to Atg8/LC3 to facilitate degradation of ubiquitinated protein aggregates by autophagy"**
*作者：Pankiv S, Clausen TH, et al.*
摘要：该研究通过体外重组蛋白实验，证明SQSTM1的LC3相互作用区(LIR)直接结合自噬相关蛋白LC3.建立泛素化底物与自噬膜间的分子桥梁，促进聚集体清除。
**Background of SQSTM1 Recombinant Protein**
SQSTM1 (Sequestosome-1), also known as p62. is a multifunctional scaffold protein encoded by the *SQSTM1* gene. It plays a central role in cellular processes such as selective autophagy, ubiquitin-proteasome system regulation, and signal transduction. Structurally, SQSTM1 contains multiple domains, including a PB1 domain for oligomerization and protein interactions, a ZZ-type zinc finger domain, a TRAF6-binding motif, a LC3-interacting region (LIR), and a ubiquitin-associated (UBA) domain. These domains enable SQSTM1 to act as a cargo receptor for ubiquitinated substrates, shuttling them to autophagosomes for degradation via its interaction with LC3/GABARAP proteins.
SQSTM1 is critical for maintaining cellular homeostasis by regulating oxidative stress, inflammation, and nutrient sensing through pathways like NF-κB and mTORC1. Dysregulation of SQSTM1 is linked to various diseases, including neurodegenerative disorders (e.g., Alzheimer’s, ALS), cancer, and Paget’s disease of bone. Mutations in *SQSTM1* are associated with impaired autophagy and accumulation of toxic protein aggregates, highlighting its role in proteostasis.
Recombinant SQSTM1 protein is produced using expression systems (e.g., *E. coli*, mammalian cells) to study its structure-function relationships, interactions, and therapeutic potential. It serves as a tool for elucidating autophagy mechanisms, modeling diseases *in vitro*, and screening drugs targeting SQSTM1-dependent pathways. Researchers also leverage tagged versions (e.g., His, GFP) for purification, localization, and interaction studies. Its versatility makes SQSTM1 recombinant protein a valuable resource in biomedical research, particularly in understanding and treating autophagy-related pathologies.
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