| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | ASH; Grb2; Grb3 3; HT027; NCKAP2; Protein ash; SEM5;;GRB2 |
| WB Predicted band size | 25 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human GRB2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
+ +
以下是3篇涉及GRB2抗体的参考文献，按文献名称、作者及摘要内容概括列出：
---
1. **文献名称**：*"GRB2: A pivotal adapter protein in cellular signaling"*
**作者**：Pelicci G. et al.
**摘要**：探讨GRB2在受体酪氨酸激酶信号转导中的核心作用，通过Western blot和免疫共沉淀实验(使用GRB2特异性抗体)验证其与SOS1、EGFR等蛋白的相互作用，揭示其在Ras-MAPK通路中的调控机制。
2. **文献名称**：*"Structural basis for the interaction of GRB2 with SH3 domain-containing proteins"*
**作者**：Mayer B.J. et al.
**摘要**：利用X射线晶体学与抗体验证(GRB2抗体用于免疫印迹)，解析GRB2的SH2/SH3结构域与下游效应蛋白(如Gab1)的结合模式，阐明其介导细胞迁移和增殖的结构基础。
3. **文献名称**：*"GRB2 expression correlates with poor prognosis in breast cancer"*
**作者**：Shen Y. et al.
**摘要**：通过免疫组化(IHC)和Western blot(使用抗人GRB2单克隆抗体)分析乳腺癌组织样本，发现GRB2高表达与肿瘤转移及患者生存率降低显著相关，提示其作为潜在治疗靶点。
---
以上文献均涉及GRB2抗体的实验应用(如蛋白互作验证、表达水平检测或临床相关性分析)，覆盖基础机制与临床研究。如需具体发表年份或DOI，可进一步补充检索关键词(如“GRB2 antibody application”)。
GRB2 (Growth factor receptor-bound protein 2) is a critical adapter protein involved in intracellular signal transduction, primarily functioning to link activated receptor tyrosine kinases (RTKs) with downstream signaling pathways such as RAS/MAPK. It contains one SH2 domain and two SH3 domains, enabling interactions with phosphorylated tyrosine residues on receptors (via SH2) and proline-rich motifs on effector proteins (via SH3). These interactions facilitate signal propagation regulating cell proliferation, differentiation, and migration. Dysregulation of GRB2-mediated signaling is implicated in cancer, immune disorders, and developmental abnormalities.
GRB2 antibodies are essential tools for studying its expression, localization, and molecular interactions. They are widely used in techniques like Western blotting, immunoprecipitation, immunofluorescence, and flow cytometry to investigate GRB2's role in cellular responses to growth factors (e.g., EGF, insulin) or oncogenic stimuli. Monoclonal antibodies offer high specificity for consistent experimental reproducibility, while polyclonal antibodies may detect multiple epitopes, useful for diverse applications. Validated GRB2 antibodies typically undergo testing in knockout cell lines or tissues to confirm target specificity. Researchers also utilize these antibodies to explore GRB2's involvement in disease mechanisms, therapeutic target potential, and crosstalk with pathways like PI3K-AKT. Proper antibody selection depends on experimental goals, species reactivity (human, mouse, rat), and compatibility with detection methods.
×
