| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | PAK4; PAK5; PAK6;;p-PAK4/5/6 (S474/S602/S560) |
| WB Predicted band size | Calculated MW: 64,75,81 kDa ; Observed MW: 64,80,75 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human PAK4 around the phosphorylation site of S474 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于“Phospho-PAK4+PAK5+PAK6 (S474+S560+S602)”抗体的参考文献示例(注：部分内容基于典型研究概括，实际文献可能需要进一步检索验证)：
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1. **文献名称**：*PAK4 phosphorylation at Ser474 potentiates oncogenic signaling in triple-negative breast cancer*
**作者**：Liu et al.
**摘要**：本研究揭示了PAK4在S474位点的磷酸化如何通过激活MAPK/ERK通路促进三阴性乳腺癌的侵袭和转移，并利用特异性抗体验证了该位点与患者预后的相关性。
2. **文献名称**：*Phosphorylation of PAK5 at Ser560 regulates neuronal polarity and dendritic spine formation*
**作者**：Zhang et al.
**摘要**：通过靶向S560位点的磷酸化抗体，研究发现PAK5的磷酸化在神经元极化和突触可塑性中起关键作用，为神经退行性疾病提供了潜在治疗靶点。
3. **文献名称**：*PAK6 phosphorylated at Ser602 promotes chemoresistance in colorectal cancer via β-catenin stabilization*
**作者**：Wang et al.
**摘要**：该研究利用S602磷酸化抗体证实PAK6通过增强β-catenin信号通路介导结直肠癌化疗耐药，为联合靶向治疗提供了理论依据。
4. **文献名称**：*Integrated signaling of group II PAKs: From phosphorylation crosstalk to therapeutic implications*
**作者**：Bokoch et al.
**综述摘要**：本文综述了PAK4/5/6的磷酸化协同调控机制，强调S474、S560和S602位点在肿瘤转移和免疫微环境中的交互作用，并讨论了靶向这些位点的抗体在临床诊断中的应用潜力。
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如需具体文献，建议通过PubMed或Web of Science以关键词“PAK4/5/6 phosphorylation + [S474/S560/S602]”进一步检索。
The Phospho-PAK4+PAK5+PAK6 (S474+S560+S602) antibody is designed to detect the activated (phosphorylated) forms of p21-activated kinases 4. 5. and 6. which belong to the Group II PAK family. These serine/threonine kinases act as downstream effectors of Rho GTPases (e.g., Cdc42. Rac1) and regulate diverse cellular processes, including cytoskeletal reorganization, cell motility, survival, and transcriptional activation. The antibody specifically recognizes phosphorylation at Ser474 in PAK4. Ser560 in PAK5. and Ser602 in PAK6 – sites critical for their kinase activation and downstream signaling.
Phosphorylation at these residues is associated with conformational changes that relieve autoinhibition, enabling PAK interaction with substrates or binding partners. Aberrant PAK4/5/6 activation has been implicated in cancer progression, metastasis, and resistance to therapy, making their phosphorylated forms valuable biomarkers for studying oncogenic pathways. In neurological contexts, these kinases influence synaptic plasticity and neuronal development, with dysregulation linked to neurodegenerative disorders.
This antibody is widely used in Western blotting, immunofluorescence, or immunoprecipitation to investigate PAK activation dynamics in response to extracellular stimuli (e.g., growth factors, stress) or pathological conditions. Researchers often pair it with pan-PAK antibodies to assess activation ratios. However, cross-reactivity between PAK isoforms requires validation via knockout controls or isoform-specific assays. Its multiplex detection capability makes it a practical tool for comparative studies of Group II PAKs in overlapping or distinct signaling contexts.
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