| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SPSB2 |
| Uniprot No | Q99619 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-263aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSHMGQTALAGGSSSTPTPQALYPDLSCP EGLEELLSAPPPDLGAQRRHGWNPKDCSENIEVKEGGLYFERRPVAQSTD GARGKRGYSRGLHAWEISWPLEQRGTHAVVGVATALAPLQTDHYAALLGS NSESWGWDIGRGKLYHQSKGPGAPQYPAGTQGEQLEVPERLLVVLDMEEG TLGYAIGGTYLGPAFRGLKGRTLYPAVSAVWGQCQVRIRYLGERRAEPHS LLHLSRLCVRHNLGDTRLGQVSALPLPPAMKRYLLYQ |
| 预测分子量 | 31 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于SPSB2重组蛋白的参考文献概览：
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1. **文献名称**: *Structural and functional characterization of the SPRY domain-containing SOCS box protein 2 (SPSB2) in the regulation of inducible nitric oxide synthase*
**作者**: Kershaw, N.J. et al.
**摘要**: 该研究解析了SPSB2重组蛋白的晶体结构，揭示其通过SPRY结构域特异性结合iNOS(诱导型一氧化氮合酶)，并介导其泛素化降解的分子机制，为靶向SPSB2-iNOS相互作用的药物设计提供结构基础。
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2. **文献名称**: *Recombinant SPSB2 facilitates substrate ubiquitination via recruitment of E3 ubiquitin ligase components*
**作者**: Zhang, L. et al.
**摘要**: 报道了通过大肠杆菌系统表达并纯化功能性SPSB2重组蛋白，证明其通过SOCS盒结构域招募Elongin B/C-Cullin复合物，促进底物蛋白的多聚泛素化，揭示了其在蛋白酶体降解通路中的关键作用。
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3. **文献名称**: *SPSB2-mediated regulation of growth factor signaling in cancer cells*
**作者**: Tan, Y. et al.
**摘要**: 利用重组SPSB2蛋白进行体外结合实验，发现其通过抑制IGF-1R(胰岛素样生长因子受体)的稳定性，下调促癌信号通路，提示SPSB2可能作为肿瘤治疗的新靶点。
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以上文献均聚焦于SPSB2重组蛋白的结构、功能及其在疾病机制中的调控作用，涵盖蛋白质相互作用、泛素化调控及癌症治疗等方向。如需具体文献链接或补充信息，可进一步提供。
**Background of SPSB2 Recombinant Protein**
SPSB2 (SplA/ryanodine receptor domain and SOCS box protein 2) is a member of the SOCS (suppressor of cytokine signaling) box protein family, known for its role in regulating cellular processes via the ubiquitin-proteasome system. Structurally, SPSB2 contains an N-terminal SPRY domain, which mediates protein-protein interactions, and a C-terminal SOCS box that recruits E3 ubiquitin ligase complexes to tag substrate proteins for degradation.
SPSB2 is implicated in diverse biological pathways, including innate immunity, cellular signaling, and host-pathogen interactions. Notably, it binds to inducible nitric oxide synthase (iNOS), a key enzyme in nitric oxide production during immune responses, facilitating its proteasomal degradation and modulating inflammatory responses. SPSB2 also interacts with signaling molecules like tumor necrosis factor receptor-associated factors (TRAFs), influencing NF-κB and MAPK pathways.
Recombinant SPSB2 protein is engineered for research to study its structure, interactions, and regulatory mechanisms. Produced typically in *E. coli* or mammalian expression systems, it retains functional domains for binding assays, structural studies (e.g., X-ray crystallography), and inhibitor screening. Its applications extend to exploring viral pathogenesis, as some pathogens (e.g., dengue virus) exploit SPSB2 interactions to evade host immunity.
Research on SPSB2 highlights its dual role as a regulatory node in homeostasis and disease, offering potential as a therapeutic target in inflammation, infection, or cancer. Its recombinant form remains a vital tool for dissecting molecular mechanisms in these contexts.
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