| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SPSB1 |
| Uniprot No | Q96BD6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 24-233aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMQELQGLDYCKPTRLDLLLDMPPVSYDVQL LHSWNNNDRSLNVFVKEDDKLIFHRHPVAQSTDAIRGKVGYTRGLHVWQI TWAMRQRGTHAVVGVATADAPLHSVGYTTLVGNNHESWGWDLGRNRLYHD GKNQPSKTYPAFLEPDETFIVPDSFLVALDMDDGTLSFIVDGQYMGVAFR GLKGKKLYPVVSAVWGHCEIRMRYLNGLDPE |
| 预测分子量 | 26 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SPSB1重组蛋白的3篇代表性文献示例(注：部分信息为模拟概括，建议通过学术数据库核实具体内容)：
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1. **文献名称**：*Structural basis for the interaction of the SPRY domain of human SPSB1 with inducible nitric oxide synthase*
**作者**：D.J. Marc et al.
**摘要**：本研究通过X射线晶体学解析了SPSB1重组蛋白的SPRY结构域与诱导型一氧化氮合酶(iNOS)的复合物结构，揭示了SPSB1通过结合iNOS的特定肽段促进其泛素化降解的分子机制，为靶向该相互作用的药物设计提供结构基础。
2. **文献名称**：*Recombinant SPSB1 regulates cytokine signaling by targeting iNOS in macrophages*
**作者**：N.A. de Silva et al.
**摘要**：作者利用大肠杆菌表达系统纯化重组SPSB1蛋白，发现其通过SPRY结构域特异性结合iNOS，抑制巨噬细胞中一氧化氮(NO)的过量产生，表明SPSB1在炎症调控中具有潜在作用。
3. **文献名称**：*SPSB1 promotes tumor progression via ubiquitination-dependent pathways in colorectal cancer*
**作者**：H.L. Zhang et al.
**摘要**：该研究通过体外重组SPSB1蛋白实验证实，SPSB1通过招募E3泛素连接酶复合物，促进肿瘤抑制因子的降解，从而增强结直肠癌细胞增殖和转移能力，提示其作为癌症治疗靶点的潜力。
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**注意**：以上文献信息为示例性质，实际研究中请通过PubMed、Web of Science等平台检索真实文献(关键词：SPSB1 recombinant protein, SPRY domain, ubiquitination)。
SPSB1 (SPRY domain-containing SOCS box protein 1) is a member of the SPSB protein family characterized by two conserved structural motifs: an N-terminal SPRY domain and a C-terminal SOCS box. These proteins are implicated in regulating protein-protein interactions and ubiquitin-mediated proteasomal degradation. The SPRY domain facilitates substrate recognition by binding to specific motifs, such as the "GVM" motif in inducible nitric oxide synthase (iNOS), while the SOCS box interacts with Elongin B/C complexes, linking target proteins to E3 ubiquitin ligase machinery for polyubiquitination and subsequent degradation.
SPSB1 is predominantly expressed in immune and reproductive tissues, playing roles in cellular processes like apoptosis, proliferation, and immune response modulation. Studies highlight its involvement in infectious diseases and cancer. For instance, SPSB1-mediated degradation of iNOS in macrophages influences host defense against pathogens like *Salmonella*. In cancer, SPSB1 may act as an oncogene by stabilizing oncoproteins or suppressing tumor suppressors, though its context-dependent roles remain under investigation.
Recombinant SPSB1 protein, typically produced in *E. coli* or mammalian expression systems, retains functional domains for biochemical assays, structural studies, and inhibitor screening. It serves as a tool to decipher SPSB1-substrate interactions or develop therapeutic strategies targeting diseases linked to dysregulated ubiquitination. Recent research also explores its potential in antiviral immunity and fertility regulation, reflecting its broad biological relevance. Despite progress, mechanistic details of SPSB1's dual roles in health and disease warrant further exploration to harness its therapeutic potential.
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