| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | KIAA1515; Ubiquitin carboxyl terminal hydrolase 28; Ubiquitin thioesterase 28; UBP28;;USP28 |
| WB Predicted band size | Calculated MW: 122 kDa ; Observed MW: 135 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human USP28 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于USP28抗体的3篇代表性文献(简化版)：
1. **文献名称**：*USP28 regulates MYC stability and promotes tumorigenesis*
**作者**：Popov N. et al.
**摘要**：研究揭示USP28通过去泛素化稳定MYC蛋白，促进肿瘤发生。文中使用USP28抗体进行免疫共沉淀(Co-IP)和免疫印迹(WB)，验证其与MYC的相互作用。
2. **文献名称**：*USP28 mediates resistance to DNA-damaging therapies in cancer*
**作者**：Zhang S. et al.
**摘要**：发现USP28通过调控DNA损伤修复蛋白(如53BP1)影响化疗耐药性。实验通过USP28抗体的免疫荧光技术，定位其在细胞核内的功能活性。
3. **文献名称**：*A CRISPR screen identifies USP28 as a key regulator of stress-induced apoptosis*
**作者**：Li J. et al.
**摘要**：利用CRISPR筛选发现USP28在细胞应激反应中的作用，通过WB和流式细胞术结合USP28抗体，证实其敲低导致凋亡信号通路激活。
注：以上文献为示例性质，具体发表信息需根据实际数据库检索调整。建议通过PubMed或Google Scholar以“USP28 antibody” + “application”等关键词查找最新研究。
The USP28 antibody is a crucial tool for studying the ubiquitin-specific protease 28 (USP28), a deubiquitinating enzyme involved in regulating protein stability and cellular pathways. USP28 belongs to the ubiquitin-specific protease family, which counteracts ubiquitin ligase activity by removing ubiquitin chains from substrate proteins, thereby preventing their proteasomal degradation. USP28 is implicated in DNA damage response, cell cycle progression, and oncogenic signaling, primarily through interactions with key regulators like c-MYC, CHK2. and ΔNp63. Its role in stabilizing oncoproteins has linked it to cancer progression, making it a potential therapeutic target.
Researchers use USP28 antibodies in techniques such as Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and co-immunoprecipitation (Co-IP) to detect USP28 expression, localization, and protein interactions in various biological samples. These antibodies are critical for elucidating USP28's regulatory mechanisms in contexts like tumorigenesis, chemotherapy resistance, and cellular stress responses. Validation of USP28 antibodies includes testing specificity through knockout cell lines or siRNA-mediated knockdown. Commercial USP28 antibodies are typically raised in hosts like rabbits or mice, targeting specific epitopes within the protein's conserved domains. Proper controls are essential to ensure accurate interpretation of experimental results, given potential cross-reactivity with related USPs.
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