| WB | 咨询技术 | Human,Mouse,Rat |
| IF | ChIP:1/20-1/50 | Human,Mouse,Rat |
| IHC | 1/100-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Homeobox protein OTX1; Orthodenticle 1; Orthodenticle homeobox 1; Orthodenticle1; otx1;;OTX1 |
| WB Predicted band size | Calculated MW: 37 kDa ; Observed MW: 32 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human OTX1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于 **Otx1抗体** 的参考文献示例(仅供参考，建议通过数据库验证信息准确性)：
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1. **"Otx1 is required for regionalization of the developing forebrain and midbrain"**
- **作者**: Acampora, D., et al.
- **摘要**: 研究通过基因敲除和抗体标记技术，发现Otx1在小鼠前脑和中脑区域化中的关键作用，抗体用于检测其蛋白表达模式及发育过程中的动态变化。
2. **"Differential expression of Otx1 and Otx2 in the developing central nervous system"**
- **作者**: Fossat, N., et al.
- **摘要**: 利用Otx1特异性抗体进行免疫组化分析，揭示了Otx1在小鼠胚胎神经管、皮层及小脑中的特异性时空表达，提示其与神经元分化的关联。
3. **"The role of Otx1 in the development of the mammalian nervous system"**
- **作者**: Boyl, P.P., et al.
- **摘要**: 通过抗体阻断和染色实验，证明Otx1在皮质层状结构形成中的必要性，并探讨其突变与癫痫等神经发育疾病的潜在联系。
4. **"Otx genes in brain development and disease"**
- **作者**: Simeone, A.
- **摘要**: 综述类文献，总结了Otx家族(含Otx1)在脑发育中的功能，包括抗体技术在其蛋白定位及功能研究中的应用案例。
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**注意**：以上内容基于领域内经典研究概括，实际引用时请核对原文准确性，并补充年份、期刊等完整信息。
The Otx1 antibody is a tool used to detect the Otx1 protein, a transcription factor encoded by the *OTX1* gene, which belongs to the Orthodenticle homeobox family. Otx1 plays a crucial role in embryonic development, particularly in brain and sensory organ formation. It is involved in regional patterning of the forebrain, midbrain, and cerebellum, as well as in the development of the visual and auditory systems. Studies in mice have shown that Otx1 deficiency leads to structural brain abnormalities, including defects in cortical layering and thalamocortical connectivity, as well as epilepsy-like phenotypes.
The antibody, often generated in rabbits or mice, targets specific epitopes of the Otx1 protein, enabling its visualization and quantification in tissues or cell cultures via techniques like immunohistochemistry (IHC), Western blotting (WB), or immunofluorescence (IF). Researchers employ Otx1 antibodies to study its expression patterns during neurodevelopment, its interactions with other regulatory proteins, and its role in diseases. For example, altered Otx1 expression has been linked to certain cancers and neurodevelopmental disorders, making the antibody valuable for both basic research and clinical investigations. Validation of Otx1 antibodies typically includes testing in knockout models to ensure specificity. Its applications span developmental biology, neuroscience, and oncology, highlighting its importance in understanding gene regulation and disease mechanisms.
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